Abstract
O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.
| Original language | English |
|---|---|
| Pages (from-to) | 306-311 |
| Number of pages | 6 |
| Journal | Journal of Plant Physiology |
| Volume | 156 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 2000 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Physiology
- Agronomy and Crop Science
- Plant Science
Cite this
The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1. / Nita-Lazar, Mihai; Iwahara, Shojiro; Takegawa, Kaoru; Lienart, Yvette.
In: Journal of Plant Physiology, Vol. 156, No. 3, 01.01.2000, p. 306-311.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1
AU - Nita-Lazar, Mihai
AU - Iwahara, Shojiro
AU - Takegawa, Kaoru
AU - Lienart, Yvette
PY - 2000/1/1
Y1 - 2000/1/1
N2 - O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.
AB - O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.
UR - http://www.scopus.com/inward/record.url?scp=0034026796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034026796&partnerID=8YFLogxK
U2 - 10.1016/S0176-1617(00)80066-8
DO - 10.1016/S0176-1617(00)80066-8
M3 - Article
AN - SCOPUS:0034026796
VL - 156
SP - 306
EP - 311
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
IS - 3
ER -