TY - JOUR
T1 - The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1
AU - Nita-Lazar, Mihai
AU - Iwahara, Shojiro
AU - Takegawa, Kaoru
AU - Lienart, Yvette
N1 - Funding Information:
This work was supported in part by a grant from Region RhoneAlpes to Mihai Nita-Lazar (TEMPRA n° EO 84580002).
PY - 2000/3
Y1 - 2000/3
N2 - O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.
AB - O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.
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U2 - 10.1016/S0176-1617(00)80066-8
DO - 10.1016/S0176-1617(00)80066-8
M3 - Article
AN - SCOPUS:0034026796
VL - 156
SP - 306
EP - 311
JO - Z. PFLANZENPHYSIOL.
JF - Z. PFLANZENPHYSIOL.
SN - 0176-1617
IS - 3
ER -