The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1

Mihai Nita-Lazar, Shojiro Iwahara, Kaoru Takegawa, Yvette Lienart

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalJournal of Plant Physiology
Volume156
Issue number3
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

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Glucuronic Acid
glucuronic acid
Lipoxygenase
Respiratory Burst
Rubus
Protoplasts
raspberries
Fusarium
lipoxygenase
Superoxides
superoxide anion
protoplasts
Peroxidase
Polysaccharides
glycoproteins
Reactive Oxygen Species
Glycoproteins
peroxidase
plasma membrane
polysaccharides

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1. / Nita-Lazar, Mihai; Iwahara, Shojiro; Takegawa, Kaoru; Lienart, Yvette.

In: Journal of Plant Physiology, Vol. 156, No. 3, 01.01.2000, p. 306-311.

Research output: Contribution to journalArticle

Nita-Lazar, Mihai ; Iwahara, Shojiro ; Takegawa, Kaoru ; Lienart, Yvette. / The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1. In: Journal of Plant Physiology. 2000 ; Vol. 156, No. 3. pp. 306-311.
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