The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1

Mihai Nita-Lazar, Shojiro Iwahara, Kaoru Takegawa, Yvette Lienart

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalJournal of Plant Physiology
Volume156
Issue number3
DOIs
Publication statusPublished - Mar 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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