The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1

Mihai Nita-Lazar; Shojiro Iwahara; Kaoru Takegawa; Yvette Lienart

doi:10.1016/S0176-1617(00)80066-8

The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1

Mihai Nita-Lazar, Shojiro Iwahara, Kaoru Takegawa, Yvette Lienart

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O₂·^- and of H₂O₂, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O₂·^-dismutating in H₂O₂.

Original language	English
Pages (from-to)	306-311
Number of pages	6
Journal	Journal of Plant Physiology
Volume	156
Issue number	3
DOIs	https://doi.org/10.1016/S0176-1617(00)80066-8
Publication status	Published - Mar 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physiology
Agronomy and Crop Science
Plant Science

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10.1016/S0176-1617(00)80066-8

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title = "The active oxygen response of raspberry protoplasts to O-glycans of Fusarium sp. M7-1",

abstract = "O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.",

author = "Mihai Nita-Lazar and Shojiro Iwahara and Kaoru Takegawa and Yvette Lienart",

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AU - Nita-Lazar, Mihai

AU - Iwahara, Shojiro

AU - Takegawa, Kaoru

AU - Lienart, Yvette

PY - 2000/3

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N2 - O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.

AB - O-glycans isolated from glucuronic acid-containing glycoproteins of Fusarium sp. M7-1 had been used as a signaling molecule. The role of the oxidative burst, fast and transient production of activated oxygen species, was investigated in Rubus protoplasts. Two assays, based on Cyt c reduction and on pyranine quenching, were used to scrutinize the generation of O2·- and of H2O2, respectively, and agents known to scavenge active oxygen species or to alter the lipoxygenase pathway. The results suggested that the burst did not involve peroxidase but rather a trans-plasma membrane superoxide synthase formed O2·-dismutating in H2O2.

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