The activity of soluble VCAM-1 in angiogenesis stimulated by IL-4 and IL-13

J. I. Fukushi, M. Ono, W. Morikawa, Y. Iwamoto, M. Kuwano

Research output: Contribution to journalArticle

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Abstract

IL-13 is a multifunctional lymphokine sharing a number of biological properties with IL-4. We previously observed that IL-4 shows angiogenic activities in vitro as well as in vivo. In this study we examined the effect of IL-13 on angiogenesis in vitro and in vivo and also the underlying mechanisms. Human IL-13 significantly stimulated the formation of tube-like structures in collagen gels by human microvascular endothelial cells and bovine aortic endothelial cells by about 3-fold over the controls in the absence of the cytokines. Administration of murine IL-13 led to neovascularization when implanted in the rat cornea. Coadministration of neutralizing mAb to the IL-4R inhibited both tubular morphogenesis in vitro and activation of STAT6 induced by IL-4 or IL-13. Both IL-4 and IL-13 markedly increased mRNA levels of VCAM-1 in vascular endothelial cells, and the production of the soluble form of VCAM-1 was also stimulated in response to IL-4 or IL-13. Administration of anti-VCAM-1 Ab in vitro blocked tubular morphogenesis induced by IL-4 and IL-13. Angiogenesis induced in vivo in rat cornea by IL-4 and IL-13 was also inhibited by Ab against the rat α4 integrin subunit. These findings suggest that angiogenesis dependent on IL-4 and IL-13 is mainly mediated through a soluble VCAM-1/α4 integrin pathway.

Original languageEnglish
Pages (from-to)2818-2823
Number of pages6
JournalJournal of Immunology
Volume165
Issue number5
DOIs
Publication statusPublished - Sep 1 2000

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Interleukin-13
Vascular Cell Adhesion Molecule-1
Interleukin-4
Endothelial Cells
Morphogenesis
Integrins
Cornea
Lymphokines
Collagen
Gels
Cytokines
Messenger RNA
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Fukushi, J. I., Ono, M., Morikawa, W., Iwamoto, Y., & Kuwano, M. (2000). The activity of soluble VCAM-1 in angiogenesis stimulated by IL-4 and IL-13. Journal of Immunology, 165(5), 2818-2823. https://doi.org/10.4049/jimmunol.165.5.2818

The activity of soluble VCAM-1 in angiogenesis stimulated by IL-4 and IL-13. / Fukushi, J. I.; Ono, M.; Morikawa, W.; Iwamoto, Y.; Kuwano, M.

In: Journal of Immunology, Vol. 165, No. 5, 01.09.2000, p. 2818-2823.

Research output: Contribution to journalArticle

Fukushi, JI, Ono, M, Morikawa, W, Iwamoto, Y & Kuwano, M 2000, 'The activity of soluble VCAM-1 in angiogenesis stimulated by IL-4 and IL-13', Journal of Immunology, vol. 165, no. 5, pp. 2818-2823. https://doi.org/10.4049/jimmunol.165.5.2818
Fukushi, J. I. ; Ono, M. ; Morikawa, W. ; Iwamoto, Y. ; Kuwano, M. / The activity of soluble VCAM-1 in angiogenesis stimulated by IL-4 and IL-13. In: Journal of Immunology. 2000 ; Vol. 165, No. 5. pp. 2818-2823.
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