The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans

Yosuke Matsubara, Ichiro Kawasaki, Seiichi Urushiyama, Tomoharu Yasuda, Masaki Shirakata, Yuichi Iino, Hiroshi Shibuya, Yuji Yamanashi

Research output: Contribution to journalArticle

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Abstract

The Ras-MAP kinase pathway regulates varieties of fundamental cellular events. In Caenorhabditis elegans, this pathway is required for oocyte development; however, the nature of its up-stream regulators has remained elusive. Here, we identified a C. elegans gene, rog-1, which encodes the only protein having the IRS-type phosphotyrosine-binding (PTB) domain in the worms. ROG-1 has no obvious domain structure aside from the PTB domain, suggesting that it could serve as an adaptor down-stream of protein-tyrosine kinases (PTKs). RNA interference (RNAi)-mediated down-regulation of rog-1 mRNA significantly decreased brood size. rog-1 (tm1031) truncation mutants showed a severe disruption in progression of developing oocytes from pachytene to diakinesis, as was seen in worms carrying a loss-of-function mutation in the let-60 Ras or mpk-1 MAP kinase gene. Furthermore, let-60 Ras-regulated activation of MPK-1 in the gonad is undetectable in rog-1 (tm1031) mutants. Conversely, a gain-of-function mutation in the let-60 Ras gene rescues the brood size reduction and germ cell abnormality in rog-1 (tm1031) worms. Consistently, rog-1 is preferentially expressed in the germ cells and its expression in the gonad is essential for oocyte development. Thus, ROG-1 is a key positive regulator of the Ras-MAP kinase pathway that permits germ cells to exit from pachytene.

Original languageEnglish
Pages (from-to)407-420
Number of pages14
JournalGenes to Cells
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 1 2007

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Caenorhabditis elegans
Germ Cells
Oocytes
Cell Cycle
Phosphotyrosine
Phosphotransferases
Gonads
Meiotic Prophase I
Mutation
Proteins
ras Genes
RNA Interference
Protein-Tyrosine Kinases
Genes
Down-Regulation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans. / Matsubara, Yosuke; Kawasaki, Ichiro; Urushiyama, Seiichi; Yasuda, Tomoharu; Shirakata, Masaki; Iino, Yuichi; Shibuya, Hiroshi; Yamanashi, Yuji.

In: Genes to Cells, Vol. 12, No. 3, 01.03.2007, p. 407-420.

Research output: Contribution to journalArticle

Matsubara, Y, Kawasaki, I, Urushiyama, S, Yasuda, T, Shirakata, M, Iino, Y, Shibuya, H & Yamanashi, Y 2007, 'The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans', Genes to Cells, vol. 12, no. 3, pp. 407-420. https://doi.org/10.1111/j.1365-2443.2007.01061.x
Matsubara, Yosuke ; Kawasaki, Ichiro ; Urushiyama, Seiichi ; Yasuda, Tomoharu ; Shirakata, Masaki ; Iino, Yuichi ; Shibuya, Hiroshi ; Yamanashi, Yuji. / The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans. In: Genes to Cells. 2007 ; Vol. 12, No. 3. pp. 407-420.
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