The amino acid sequence required for 5' → 3' exonuclease activity of bacillus caldotenax DNA polymerase

Yoshizumi Ishino, Asako Takahashi-Fujii, Takashi Uemori, Mitsuo Imamura, Ikunoshin Kato

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We studied the 5' ↑ 3' exonuclease activity of Bacillus caldotenax DNA polymerase by site-directed mutagenesis. Among seven mutants constructed, two mutant DNA polymerases with an amino acid substitution of Glyl84 ↑ Asp or Glyl92 ↑ Asp were confirmed to be deficient in this exonuclease. The two positions corresponded to those of the Escherichia coli DNA polymerase I mutants defective in 5' ↑ 3' exonuclease, polA480ex and polA214. These results provide experimental support for the proposed amino acid sequence essential for the 5' ↑ 3' exonuclease activity associated with eubacterial polymerase I-like DNA polymerases (family A), including E.coli and Thermits aquaticus.

Original languageEnglish
Pages (from-to)1171-1175
Number of pages5
JournalProtein Engineering, Design and Selection
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 1 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Molecular Biology

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