The amino acid sequence required for 5' → 3' exonuclease activity of bacillus caldotenax DNA polymerase

Yoshizumi Ishino, Asako Takahashi-Fujii, Takashi Uemori, Mitsuo Imamura, Ikunoshin Kato

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We studied the 5' ↑ 3' exonuclease activity of Bacillus caldotenax DNA polymerase by site-directed mutagenesis. Among seven mutants constructed, two mutant DNA polymerases with an amino acid substitution of Glyl84 ↑ Asp or Glyl92 ↑ Asp were confirmed to be deficient in this exonuclease. The two positions corresponded to those of the Escherichia coli DNA polymerase I mutants defective in 5' ↑ 3' exonuclease, polA480ex and polA214. These results provide experimental support for the proposed amino acid sequence essential for the 5' ↑ 3' exonuclease activity associated with eubacterial polymerase I-like DNA polymerases (family A), including E.coli and Thermits aquaticus.

Original languageEnglish
Pages (from-to)1171-1175
Number of pages5
JournalProtein Engineering, Design and Selection
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 1995
Externally publishedYes

Fingerprint

Exonucleases
Bacilli
DNA-Directed DNA Polymerase
DNA Polymerase I
Bacillus
Amino acids
Amino Acid Sequence
DNA
Amino Acids
Viperidae
Escherichia coli
Mutagenesis
Amino Acid Substitution
Site-Directed Mutagenesis
Substitution reactions

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Molecular Biology
  • Bioengineering
  • Biotechnology
  • Biochemistry

Cite this

The amino acid sequence required for 5' → 3' exonuclease activity of bacillus caldotenax DNA polymerase. / Ishino, Yoshizumi; Takahashi-Fujii, Asako; Uemori, Takashi; Imamura, Mitsuo; Kato, Ikunoshin.

In: Protein Engineering, Design and Selection, Vol. 8, No. 11, 11.1995, p. 1171-1175.

Research output: Contribution to journalArticle

Ishino, Yoshizumi ; Takahashi-Fujii, Asako ; Uemori, Takashi ; Imamura, Mitsuo ; Kato, Ikunoshin. / The amino acid sequence required for 5' → 3' exonuclease activity of bacillus caldotenax DNA polymerase. In: Protein Engineering, Design and Selection. 1995 ; Vol. 8, No. 11. pp. 1171-1175.
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