The anti-atherosclerotic di-peptide, Trp-His, inhibits the phosphorylation of voltage-dependent L-type Ca 2+ channels in rat vascular smooth muscle cells

Yutaro Kobayashi, Toshihiko Fukuda, Mitsuru Tanaka, Toshiro Matsui

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Trp-His is the only vasoactive di-peptide known to regulate intracellular Ca 2+ ([Ca 2+] i) and prevent the onset of atherosclerosis in mice. In this study, we showed that Trp-His reduced the [Ca 2+] i elevation in phospholipase C-activated vascular smooth muscle cells (VSMCs), while a mixture of the corresponding constituent amino acids did not show significant reduction. Furthermore, Trp-His suppressed calmodulin-dependent kinase II (CaMK II) activity in angiotensin II-stimulated VSMCs, resulting in the inhibition of phosphorylation of voltage-dependent L-type Ca 2+ channels (VDCC). Therefore, Trp-His potentially regulates the VDCC phosphorylation cascade through Ca 2+-CaM/CaMK II.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalFEBS Open Bio
Volume2
DOIs
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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