Abstract
Trp-His, the anti-atherosclerotic dipeptide, exerted an antiproliferative effect on vascular smooth muscle cells by L-type Ca2+ channel blocker-like effect. The beneficial potential by the blockade of Ca2+ channels on chronic intestinal inflammation, including inflammatory bowel disease (IBD), is unclear. Trp-His (100 or 250 mg/kg body weight/day) was administered for 14 days to BALB/c mice, and 5% dextran sodium sulfate (DSS) was administered to induce colitis in the last 7 days. Trp-His reduced DSS-induced typical colitis symptoms and cytokine expression in the colon. Trp-His inhibited interleukin (IL)-8 secretion in tumor necrosis factor (TNF)-α-stimulated HT-29 cells. The inhibitory effect of Trp-His, as well as that of Ca2+ channel blockers, was impaired by the presence of Ca2+ channel agonist Bay K 8644. The TNF-α-induced activation of mitogen-activated protein kinases (MAPKs) and IκBα were decreased by Trp-His. These results indicated that the anti-inflammatory effect of Trp-His may be involved in the blockade of L-type Ca2+ channels.
| Original language | English |
|---|---|
| Pages (from-to) | 6041-6050 |
| Number of pages | 10 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 63 |
| Issue number | 26 |
| DOIs | |
| Publication status | Published - Jul 8 2015 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Agricultural and Biological Sciences(all)
Cite this
The Anti-atherosclerotic Dipeptide, Trp-His, Reduces Intestinal Inflammation through the Blockade of L-Type Ca2+ Channels. / Kobayashi, Yutaro; Kovacs-Nolan, Jennifer; Matsui, Toshiro; Mine, Yoshinori.
In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 26, 08.07.2015, p. 6041-6050.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Anti-atherosclerotic Dipeptide, Trp-His, Reduces Intestinal Inflammation through the Blockade of L-Type Ca2+ Channels
AU - Kobayashi, Yutaro
AU - Kovacs-Nolan, Jennifer
AU - Matsui, Toshiro
AU - Mine, Yoshinori
PY - 2015/7/8
Y1 - 2015/7/8
N2 - Trp-His, the anti-atherosclerotic dipeptide, exerted an antiproliferative effect on vascular smooth muscle cells by L-type Ca2+ channel blocker-like effect. The beneficial potential by the blockade of Ca2+ channels on chronic intestinal inflammation, including inflammatory bowel disease (IBD), is unclear. Trp-His (100 or 250 mg/kg body weight/day) was administered for 14 days to BALB/c mice, and 5% dextran sodium sulfate (DSS) was administered to induce colitis in the last 7 days. Trp-His reduced DSS-induced typical colitis symptoms and cytokine expression in the colon. Trp-His inhibited interleukin (IL)-8 secretion in tumor necrosis factor (TNF)-α-stimulated HT-29 cells. The inhibitory effect of Trp-His, as well as that of Ca2+ channel blockers, was impaired by the presence of Ca2+ channel agonist Bay K 8644. The TNF-α-induced activation of mitogen-activated protein kinases (MAPKs) and IκBα were decreased by Trp-His. These results indicated that the anti-inflammatory effect of Trp-His may be involved in the blockade of L-type Ca2+ channels.
AB - Trp-His, the anti-atherosclerotic dipeptide, exerted an antiproliferative effect on vascular smooth muscle cells by L-type Ca2+ channel blocker-like effect. The beneficial potential by the blockade of Ca2+ channels on chronic intestinal inflammation, including inflammatory bowel disease (IBD), is unclear. Trp-His (100 or 250 mg/kg body weight/day) was administered for 14 days to BALB/c mice, and 5% dextran sodium sulfate (DSS) was administered to induce colitis in the last 7 days. Trp-His reduced DSS-induced typical colitis symptoms and cytokine expression in the colon. Trp-His inhibited interleukin (IL)-8 secretion in tumor necrosis factor (TNF)-α-stimulated HT-29 cells. The inhibitory effect of Trp-His, as well as that of Ca2+ channel blockers, was impaired by the presence of Ca2+ channel agonist Bay K 8644. The TNF-α-induced activation of mitogen-activated protein kinases (MAPKs) and IκBα were decreased by Trp-His. These results indicated that the anti-inflammatory effect of Trp-His may be involved in the blockade of L-type Ca2+ channels.
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UR - http://www.scopus.com/inward/citedby.url?scp=84936806762&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b01682
DO - 10.1021/acs.jafc.5b01682
M3 - Article
C2 - 26079480
AN - SCOPUS:84936806762
VL - 63
SP - 6041
EP - 6050
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 26
ER -