The application of HRTEM techniques and nanosims to chemically and isotopically characterize Geobacter sulfurreducens surfaces

Mostafa Fayek, Satoshi Utsunomiya, Susan M. Pfiffner, David C. White, Lee R. Riciputi, Rodney C. Ewing, Lawrence M. Anovitz, Frank J. Stadermann

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bioprecipitated minerals are typically at the nanometer scale, hydrous, and beam-sensitive (i.e., can recrystallize during analysis), making them difficult to characterize using standard spectroscopic or electron-beam techniques. We have combined the ion-imaging capabilities of nanoscale secondary-ion mass spectrometry (NanoSIMS) and advanced high-resolution transmission electron microscopy (HRTEM) in order to characterize the surfaces of Geobacter sulfurreducens and the bioprecipitated uranium phases. Our results reveal the association between nutrient uptake and precipitation of uranium minerals. Biosequestration of uranium is enhanced by addition of nutrients such as acetic acid, and uranium is precipitated on the surface of the bacteria as nanocrystals of uraninite (UO 2 ). The bioprecipitation of this anhydrous U-rich phase is significant; although UO 2 is thermodynamically stable over a range of pH values (2 to 12) and oxidizing conditions [Eh 0.2 to -0.2, or log f(O 2 ) of approximately -50 to -125], thermodynamic models of inorganic systems suggest that U 6+ oxyhydroxide minerals should be stable. Our results suggest that the biofilm shielded the UO 2 from re-oxidation and that bacteria can immobilize uranium for extended periods, even under relatively oxidizing conditions in the subsurface.

Original languageEnglish
Pages (from-to)1631-1641
Number of pages11
JournalCanadian Mineralogist
Volume43
Issue number5
DOIs
Publication statusPublished - Oct 1 2005
Externally publishedYes

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Uranium
High resolution transmission electron microscopy
transmission electron microscopy
uranium
Minerals
Nutrients
Bacteria
mineral
uraninite
bacterium
ion
Biofilms
nutrient uptake
Secondary ion mass spectrometry
Acetic Acid
acetic acid
Nanocrystals
biofilm
Electron beams
mass spectrometry

All Science Journal Classification (ASJC) codes

  • Geochemistry and Petrology

Cite this

The application of HRTEM techniques and nanosims to chemically and isotopically characterize Geobacter sulfurreducens surfaces. / Fayek, Mostafa; Utsunomiya, Satoshi; Pfiffner, Susan M.; White, David C.; Riciputi, Lee R.; Ewing, Rodney C.; Anovitz, Lawrence M.; Stadermann, Frank J.

In: Canadian Mineralogist, Vol. 43, No. 5, 01.10.2005, p. 1631-1641.

Research output: Contribution to journalArticle

Fayek, Mostafa ; Utsunomiya, Satoshi ; Pfiffner, Susan M. ; White, David C. ; Riciputi, Lee R. ; Ewing, Rodney C. ; Anovitz, Lawrence M. ; Stadermann, Frank J. / The application of HRTEM techniques and nanosims to chemically and isotopically characterize Geobacter sulfurreducens surfaces. In: Canadian Mineralogist. 2005 ; Vol. 43, No. 5. pp. 1631-1641.
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