The archaeal Hjm helicase has recQ-like functions, and may be involved in repair of stalled replication fork

Ryosuke Fujikane, Hideo Shinagawa, Yoshizumi Ishino

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The archaeal Hjm is a structure-specific DNA helicase, which was originally identified in the hyperthermophilic archaeon, Pyrococcus furiosus, by in vitro screening for Holliday junction migration activity. Further biochemical analyses of the Hjm protein from P. furiosus showed that this protein preferably binds to fork-related Y-structured DNAs and unwinds their double-stranded regions in vitro, just like the E. coli RecQ protein. Furthermore, genetic analyses showed that Hjm produced in E. coli cells partially complemented the defect of functions of RecQ in a recQ mutant E. coli strain. These results suggest that Hjm may be a functional counterpart of RecQ in Archaea, in which it is necessary for the maintenance of genome integrity, although the amino acid sequences are not conserved. The functional interaction of Hjm with PCNA for its helicase activity further suggests that the Hjm works at stalled replication forks, as a member of the reconstituted replisomes to restart replication.

Original languageEnglish
Pages (from-to)99-110
Number of pages12
JournalGenes to Cells
Volume11
Issue number2
DOIs
Publication statusPublished - Feb 1 2006

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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