TY - JOUR
T1 - The arcuate nucleus as a primary site of satiety effect of leptin in rats
AU - Satoh, Noriko
AU - Ogawa, Yoshihiro
AU - Katsuura, Goro
AU - Hayase, Minoru
AU - Tsuji, Tetsuo
AU - Imagawa, Keiichi
AU - Yoshimasa, Yasunao
AU - Nishi, Shigeo
AU - Hosoda, Kiminori
AU - Nakao, Kazuwa
N1 - Funding Information:
We would like to thank Ms. C. Ishibashi for her excellent technical assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare, Yamanouchi Foundation for Research on Metabolic Disorders, and a grant for diabetes research for Otsuka Pharmaceutical Co., Ltd. (Tokushima, Japan).
PY - 1997
Y1 - 1997
N2 - The obese (ob) gene encodes a fat cell-derived circulating satiety factor (leptin) that is involved in the regulation of energy homeostasis. In the present study, we examined effects of i.c.v. injection of recombinant human leptin on food intake and body weight gain in rats. We also studied effects of direct microinjections of leptin into the arcuate nucleus (Arc), ventromedial hypothalamus (VMH), and lateral hypothalamus (LH). A single i.c.v. injection of recombinant human leptin (0.25-2.0 μg/rat) reduced significantly and dose-dependently food intake and body weight gain in rats. Microinjections (0.125-0.5 μg/site) into the bilateral Arc, VMH, and LH caused dose-related decreases in food intake and body weight gain as compared with vehicle-treated groups with a rank order of potency; Arc > VMH = LH. The present study provides the first direct evidence that the Arc is a primary site of satiety effect of leptin.
AB - The obese (ob) gene encodes a fat cell-derived circulating satiety factor (leptin) that is involved in the regulation of energy homeostasis. In the present study, we examined effects of i.c.v. injection of recombinant human leptin on food intake and body weight gain in rats. We also studied effects of direct microinjections of leptin into the arcuate nucleus (Arc), ventromedial hypothalamus (VMH), and lateral hypothalamus (LH). A single i.c.v. injection of recombinant human leptin (0.25-2.0 μg/rat) reduced significantly and dose-dependently food intake and body weight gain in rats. Microinjections (0.125-0.5 μg/site) into the bilateral Arc, VMH, and LH caused dose-related decreases in food intake and body weight gain as compared with vehicle-treated groups with a rank order of potency; Arc > VMH = LH. The present study provides the first direct evidence that the Arc is a primary site of satiety effect of leptin.
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U2 - 10.1016/S0304-3940(97)00163-8
DO - 10.1016/S0304-3940(97)00163-8
M3 - Article
C2 - 9131658
AN - SCOPUS:0030894113
SN - 0304-3940
VL - 224
SP - 149
EP - 152
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -