The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton

Paul A. Randazzo, Josefa Andrade, Koichi Miura, Megan T. Brown, Ya Qiu Long, Stacey Stauffer, Peter Roller, Jonathan A. Cooper

Research output: Contribution to journalArticlepeer-review

159 Citations (Scopus)

Abstract

family GTP-binding proteins are best characterized as regulators of membrane traffic, but recent studies indicate an additional role in cytoskeletal organization. An Arf GTPase-activating protein of the centaurin β family, ASAP1 (also known as centaurin β4), binds Arf and two other known regulators of the actin cytoskeleton, the tyrosine kinase Src and phosphatidylinositol 4,5-bisphosphate. In this paper, we show that ASAP1 localizes to focal adhesions and cycles with focal adhesion proteins when cells are stimulated to move. Overexpression of ASAP1 altered the morphology of focal adhesions and blocked both cell spreading and formation of dorsal ruffles induced by platelet-derived growth factor (PDGF). On the other hand, ASAP1, with a mutation that disrupted GTPase-activating protein activity, had a reduced effect on cell spreading and increased the number of cells forming dorsal ruffles in response to PDGF. These data support a role for an Arf GTPase-activating protein, ASAP1, as a regulator of cytoskeletal remodeling and raise the possibility that the Arf pathway is a target for PDGF signaling.

Original languageEnglish
Pages (from-to)4011-4016
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number8
DOIs
Publication statusPublished - Apr 11 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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