The balance of forces generated by kinesins controls spindle polarity and chromosomal heterogeneity in tetraploid cells

Sei Shu, Makoto Iimori, Takeshi Wakasa, Koji Ando, Hiroshi Saeki, Yoshinao Oda, Eiji Oki, Yoshihiko Maehara

Research output: Contribution to journalArticle

Abstract

Chromosomal instability, one of the most prominent features of tumour cells, causes aneuploidy. Tetraploidy is thought to be an intermediate on the path to aneuploidy, but the mechanistic relationship between the two states is poorly understood. Here, we show that spindle polarity (e.g. bipolarity or multipolarity) in tetraploid cells depends on the level of functional phosphorylated Eg5, a mitotic kinesin, localised to the spindle. Multipolar spindles are formed in cells with high levels of phosphorylated Eg5. This process is suppressed by inhibition of Eg5 or expression of a non-phosphorylatable Eg5 mutant, as well as by changing the balance between opposing forces required for centrosome separation. Tetraploid cells with high levels of functional Eg5 give rise to a heterogeneous aneuploid population through multipolar division, whereas cells with low levels of functional Eg5 continue to undergo bipolar division and remain tetraploid. Furthermore, Eg5 protein levels correlate with ploidy status in tumour specimens. We provide a novel explanation for the tetraploid intermediate model, i.e. spindle polarity and subsequent tetraploid cell behaviour are determined by the balance of forces generated by mitotic kinesins at the spindle.

Original languageEnglish
JournalJournal of cell science
Volume132
Issue number24
DOIs
Publication statusPublished - Dec 13 2019

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Kinesin
Tetraploidy
Aneuploidy
Centrosome
Chromosomal Instability
Ploidies
Cell Division
Neoplasms
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

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The balance of forces generated by kinesins controls spindle polarity and chromosomal heterogeneity in tetraploid cells. / Shu, Sei; Iimori, Makoto; Wakasa, Takeshi; Ando, Koji; Saeki, Hiroshi; Oda, Yoshinao; Oki, Eiji; Maehara, Yoshihiko.

In: Journal of cell science, Vol. 132, No. 24, 13.12.2019.

Research output: Contribution to journalArticle

Shu, Sei ; Iimori, Makoto ; Wakasa, Takeshi ; Ando, Koji ; Saeki, Hiroshi ; Oda, Yoshinao ; Oki, Eiji ; Maehara, Yoshihiko. / The balance of forces generated by kinesins controls spindle polarity and chromosomal heterogeneity in tetraploid cells. In: Journal of cell science. 2019 ; Vol. 132, No. 24.
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