The C. elegans ceh-36 Gene Encodes a Putative Homemodomain Transcription Factor Involved in Chemosensory Functions of ASE and AWC Neurons

Makoto Koga, Yasumi Ohshima

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Chemotaxis to water-soluble chemicals such as sodium ion is an important behavior of Caenorhabditis elegans for seeking food, and ASE chemosensory neurons have a major role in this behavior. We isolated mutants defective in chemotaxis to sodium acetate. We show here that among them ks86 had a mutation in the ceh-36 gene. ceh-36gfp reporter constructs were expressed in ASE and AWC neurons. In a mutant of the che-1 gene, which encodes another transcription factor and is required for specification of ASE neurons, expression of the ceh-36gfp reporter in ASE is lost. This indicates that the ceh-36 gene functions downstream of the che-1 gene in ASE. In the ceh-36(ks86) mutant, expression of the tax-2 gene encoding a cyclic nucleotide-gated channel was reduced in ASE and AWC. This affords an explanation for defects of the ceh-36 mutant in the chemotaxis mediated by ASE and AWC. When a ceh-36 cDNA was expressed in an adult ceh-36 mutant by a heat shock promoter, chemotaxis to sodium acetate was recovered. These results suggest that ceh-36 is required for functions, and not for development, of ASE.

Original languageEnglish
Pages (from-to)579-587
Number of pages9
JournalJournal of Molecular Biology
Volume336
Issue number3
DOIs
Publication statusPublished - Feb 20 2004

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Chemotaxis
Transcription Factors
Neurons
Sodium Acetate
Genes
pX Genes
Cyclic Nucleotide-Gated Cation Channels
Caenorhabditis elegans
Shock
Complementary DNA
Hot Temperature
Sodium
Ions
Food
Mutation
Water

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

The C. elegans ceh-36 Gene Encodes a Putative Homemodomain Transcription Factor Involved in Chemosensory Functions of ASE and AWC Neurons. / Koga, Makoto; Ohshima, Yasumi.

In: Journal of Molecular Biology, Vol. 336, No. 3, 20.02.2004, p. 579-587.

Research output: Contribution to journalArticle

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