TY - JOUR
T1 - The C. elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell
AU - Kagoshima, Hiroshi
AU - Sawa, Hitoshi
AU - Mitani, Shohei
AU - Bürglin, Thomas R.
AU - Shigesada, Katsuya
AU - Kohara, Yuji
N1 - Funding Information:
We thank Akiko Kamamoto and Junko Kajiwara for excellent technical help, Patrick Dessi for critical reading of the manuscript, Yoshihiro Miwa for useful discussion and suggestions, Yukinobu Arata for technical advice, Joohong Ahnn, Rachael Nimmo and Alison Woollard for communicating unpublished data, Andrew Fire, Min Han and Takeshi Ishihara for useful vectors and plasmids, Judith Kimble and Ambrose R Kidd III for a GFP∷POP-1 strain and its reporter construct, and members of Kohara lab for comments and discussion on the manuscript. We should also like to thank WormBase for providing us with an ideal environment for bioinformatics. Many strains are provided by the Caenorhabditis elegans Genetic Center, which is funded by the NIH. T.R.B. received support from a START fellowship by the SNF and NF. 3130–038786.93 and is now supported by a grant from the Swedish Foundation for Strategic Research. This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan to Y.K.
PY - 2005/11/15
Y1 - 2005/11/15
N2 - The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases. Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis. C. elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1::GFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages. rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that rnt-1 is allelic to mab-2. Mutant analysis suggested that rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Wnt signaling pathway. Expression studies of GFP::POP-1 and TLP-1::GFP reporters in rnt-1/mab-2 mutants indicated that this gene functions upstream of tlp-1 and downstream, or in parallel to, pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/MAB-2 functions with POP-1 to control the asymmetry of the T cell division.
AB - The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases. Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis. C. elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1::GFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages. rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that rnt-1 is allelic to mab-2. Mutant analysis suggested that rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Wnt signaling pathway. Expression studies of GFP::POP-1 and TLP-1::GFP reporters in rnt-1/mab-2 mutants indicated that this gene functions upstream of tlp-1 and downstream, or in parallel to, pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/MAB-2 functions with POP-1 to control the asymmetry of the T cell division.
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U2 - 10.1016/j.ydbio.2005.08.034
DO - 10.1016/j.ydbio.2005.08.034
M3 - Article
C2 - 16226243
AN - SCOPUS:27744489448
VL - 287
SP - 262
EP - 273
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -
