The cationic cell-wall-peroxidase having oxidation ability for polymeric substrate participates in the late stage of lignification of Populus alba L

Shinya Sasaki, Kei'ichi Baba, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Previously we reported that purified Cell Wall Peroxidase-Cationic (CWPO-C) from poplar callus (Populus alba L.) oxidizes sinapyl alcohol and polymeric substrate unlike other plant peroxidases and proposed that this isoenzyme is a conceivable lignification specific peroxidase. In this study, we cloned full-length cDNA of CWPO-C and investigated the transcription of CWPO-C gene in various organs and the localization of CWPO-C protein in the differentiating xylem of poplar stem. Real-time PCR analyses indicated that CWPO-C gene is constitutively expressed in the developing xylem, leaf, and shoot but not affected by many stress treatments. Immunohistochemical analysis showed that CWPO-C locates in the middle lamellae, cell corners, and secondary cell walls of the fiber cells during the lignification. The intensity of the CWPO-C labeling increased gradually from the cell wall thickening stage to mature stage of fiber cells, which is very consistent with the increase of lignin content in the developing xylem. These results strongly support that CWPO-C is responsible for the lignification of the secondary xylem. Interestingly, immuno-labeling of CWPO-C was also observed inside of the ray parenchyma cells instead no signals were detected within the developing fiber cells. This suggests that CWPO-C is biosynthesized in the parenchyma cells and provided to the middle lamellae, the cell corners, and the cell walls to achieve lignin polymerization.

Original languageEnglish
Pages (from-to)797-807
Number of pages11
JournalPlant Molecular Biology
Volume62
Issue number6
DOIs
Publication statusPublished - Dec 1 2006

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Populus
Populus alba
lignification
Cell Wall
Peroxidase
peroxidase
cell walls
oxidation
Xylem
fiber cells
xylem
Lignin
parenchyma (plant tissue)
lignin
sinapyl alcohol
cells
Peroxidases
secondary xylem
peroxidases
Bony Callus

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

The cationic cell-wall-peroxidase having oxidation ability for polymeric substrate participates in the late stage of lignification of Populus alba L. / Sasaki, Shinya; Baba, Kei'ichi; Nishida, Tomoaki; Tsutsumi, Yuji; Kondo, Ryuichiro.

In: Plant Molecular Biology, Vol. 62, No. 6, 01.12.2006, p. 797-807.

Research output: Contribution to journalArticle

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