The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster

Hayashida Toshiro, Takeshi Sekiguchi, Noguchi Eishi, Sunamoto Hidetoshi, Ohba Tomoyuki, Nishimoto Takeharu

Research output: Contribution to journalArticle

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Abstract

The CCG1 cDNA encoding a general transcription factor, TAFII250, complements a thermosensitive (ts) cell cycle mutant, tsBN462, of the BHK21 cell line, which arrests in the G1 phase at the restrictive temperature. In order to clarify whether the CCG1 is mutated in tsBN462 cells or a suppressor of tsBN462 mutation, CCG1 cDNAs isolated from parental wild-type (wt) BHK21 and tsBN462 cell lines were sequenced. Comparison of the nucleotide (nt) sequences showed a single transition: G→A in the second base of codon 690 of the tsBN462 CCG1 cDNA, resulting in a Gly290→Asp change. The BHK CCG1 cDNA, but not the tsBN462 CCG1 cDNA, complemented the tsBN462 mutation, proving that the CCG1 is mutated in the tsBN462 cell line. Thus, the defect of general transcription factor, TAFII250, is suggested to cause a G1 arrest in the cell cycle.

Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalGene
Volume141
Issue number2
DOIs
Publication statusPublished - Apr 20 1994

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Mesocricetus
Complementary DNA
Cell Line
General Transcription Factors
Genes
Genetic Suppression
G1 Phase Cell Cycle Checkpoints
G1 Phase
Codon
Cell Cycle
Mutation
Temperature

All Science Journal Classification (ASJC) codes

  • Genetics

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The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster. / Toshiro, Hayashida; Sekiguchi, Takeshi; Eishi, Noguchi; Hidetoshi, Sunamoto; Tomoyuki, Ohba; Takeharu, Nishimoto.

In: Gene, Vol. 141, No. 2, 20.04.1994, p. 267-270.

Research output: Contribution to journalArticle

Toshiro, Hayashida ; Sekiguchi, Takeshi ; Eishi, Noguchi ; Hidetoshi, Sunamoto ; Tomoyuki, Ohba ; Takeharu, Nishimoto. / The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster. In: Gene. 1994 ; Vol. 141, No. 2. pp. 267-270.
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abstract = "The CCG1 cDNA encoding a general transcription factor, TAFII250, complements a thermosensitive (ts) cell cycle mutant, tsBN462, of the BHK21 cell line, which arrests in the G1 phase at the restrictive temperature. In order to clarify whether the CCG1 is mutated in tsBN462 cells or a suppressor of tsBN462 mutation, CCG1 cDNAs isolated from parental wild-type (wt) BHK21 and tsBN462 cell lines were sequenced. Comparison of the nucleotide (nt) sequences showed a single transition: G→A in the second base of codon 690 of the tsBN462 CCG1 cDNA, resulting in a Gly290→Asp change. The BHK CCG1 cDNA, but not the tsBN462 CCG1 cDNA, complemented the tsBN462 mutation, proving that the CCG1 is mutated in the tsBN462 cell line. Thus, the defect of general transcription factor, TAFII250, is suggested to cause a G1 arrest in the cell cycle.",
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