The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats

Jonas Lidholm, Alfred Szmidt, Jan Erik Hällgren, Petter Gustafsson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Chloroplast DNA from species of five different conifer genera was extracted and studied by Southern blot analysis. For all these species, hybridization with heterologous probes specific for 16 S and 23 S rDNA detected only one chloroplast DNA fragment per enzyme digest. This observation suggests that the 16 S and 23 S rRNA genes are not duplicated in these genomes. The unique 16 S rDNA-containing BamHI fragment from Pinus contorta Dougl. was cloned and restriction mapped. Apart from the 16 S rRNA gene, this fragment also contained the psbC and psbD genes. It is concluded that the chloroplast genomes of a wide taxonomic range of conifers lack one of the inverted repeat elements and that a dislocation of the psbDC gene cluster has occurred in P. contorta.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalMGG Molecular & General Genetics
Volume212
Issue number1
DOIs
Publication statusPublished - Apr 1 1988

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Chloroplast Genome
Coniferophyta
Chloroplast DNA
Ribosomal DNA
rRNA Genes
Pinus
Multigene Family
Southern Blotting
Genome
Enzymes
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats. / Lidholm, Jonas; Szmidt, Alfred; Hällgren, Jan Erik; Gustafsson, Petter.

In: MGG Molecular & General Genetics, Vol. 212, No. 1, 01.04.1988, p. 6-10.

Research output: Contribution to journalArticle

Lidholm, Jonas ; Szmidt, Alfred ; Hällgren, Jan Erik ; Gustafsson, Petter. / The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats. In: MGG Molecular & General Genetics. 1988 ; Vol. 212, No. 1. pp. 6-10.
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