The clamp-loading complex for processive DNA replication

Tomoko Miyata, Takuji Oyama, Kouta Mayanagi, Sonoko Ishino, Yoshizumi Ishino, Kosuke Morikawa

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

DNA polymerase requires two processing factors, sliding clamps and clamp loaders, to direct rapid and accurate duplication of genomic DNA. In eukaryotes, proliferating cell nuclear antigen (PCNA), the ring-shaped sliding clamp, encircles double-stranded DNA within its central hole and tethers the DNA polymerases onto DNA. Replication factor C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands in an ATP-dependent manner. Here we report the three-dimensional structure of an archaeal clamp-loading complex (RFC-PCNA-DNA) determined by single-particle EM. The three-dimensional structure of the complex, reconstituted in vitro using a nonhydrolyzable ATP analog, reveals two components, a closed ring and a horseshoe-shaped element, which correspond to PCNA and RFC, respectively. The atomic structure of PCNA fits well into the closed ring, suggesting that this ternary complex represents a state just after the PCNA ring has closed to encircle the DNA duplex.

Original languageEnglish
Pages (from-to)632-636
Number of pages5
JournalNature Structural and Molecular Biology
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 1 2004

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DNA Replication
Proliferating Cell Nuclear Antigen
Replication Protein C
DNA
DNA-Directed DNA Polymerase
Adenosine Triphosphate
Eukaryota

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

The clamp-loading complex for processive DNA replication. / Miyata, Tomoko; Oyama, Takuji; Mayanagi, Kouta; Ishino, Sonoko; Ishino, Yoshizumi; Morikawa, Kosuke.

In: Nature Structural and Molecular Biology, Vol. 11, No. 7, 01.07.2004, p. 632-636.

Research output: Contribution to journalArticle

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