The clinical evaluation of high risk human papillomavirus (HPV) genotyping in cervical cancer tissue using a novel electric DNA chip-based diagnostic system

Yui Koga, Michiyo Urata, Takeshi Uchiumi, Osamu Sato, Hiroaki Kobayashi, Dongchon Kang

Research output: Contribution to journalArticle

Abstract

Continuous infection of human papillomavirus (HPV) is known to be the major cause of cervical carcinoma. So far, more than 100 different HPV genotypes have been identified, and more than 13 kinds of HPV are known as high risk types of cervical cancer. Recently, an electric DNA chip-based diagnostic system which detects 13 HPV genotypes easily and quickly was developed. Using the electric DNA chip-based diagnostic system we performed the high risk HPV genotyping in 60 cervical cancer tissue sections and identified HPV DNA in 52 samples (86.7%). This result is consistent with the previous reports that detected the 13 high risk types of HPV from more than 90% of cervical tumor tissues. Among the HPV positive samples, HPV-16 was the most (71.2%[37cases/52cases]) and HPV-18 was the second (30.8%[16/52]) common types, followed by HPV-58(13.5%[7/52]) and -52(9.6%[5/52]). HPV-58 and -52 are prevalent HPV types in Asia and our result is in agreement with this information. There were also eight HPV-negative samples in this study, including one case with minimal deviation adenocarcinoma that is considered to develop without HPV infection. Although the HPV vaccines, such as bivalent and quadrivalent vaccines are commonly used in these days, we observed 16 cases which may not be protected by these vaccines, suggesting that these vaccines do not always protect HPV-induced carcinogenesis.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalJapanese Journal of Clinical Chemistry
Volume42
Issue number2
Publication statusPublished - Apr 1 2013

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Oligonucleotide Array Sequence Analysis
Uterine Cervical Neoplasms
Vaccines
Tissue
DNA
Papillomavirus Vaccines
Tumors
Papillomavirus Infections
Genotype
Human papillomavirus 18
Human papillomavirus 16
Carcinogenesis
Adenocarcinoma

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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The clinical evaluation of high risk human papillomavirus (HPV) genotyping in cervical cancer tissue using a novel electric DNA chip-based diagnostic system. / Koga, Yui; Urata, Michiyo; Uchiumi, Takeshi; Sato, Osamu; Kobayashi, Hiroaki; Kang, Dongchon.

In: Japanese Journal of Clinical Chemistry, Vol. 42, No. 2, 01.04.2013, p. 140-145.

Research output: Contribution to journalArticle

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