The clock gene cryptochrome of Bactrocera cucurbitae (Diptera: Tephritidae) in strains with different mating times

T. Fuchikawa, S. Sanada, R. Nishio, A. Matsumoto, T. Matsuyama, M. Yamagishi, K. Tomioka, Teiichi Tanimura, T. Miyatake

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Differences in mating time between populations can give rise to premating reproductive isolation. Tephritid fruit flies exhibit large variation in mating time among intra- or inter-specific populations. We previously cloned the clock gene period from two strains of melon fly, Bactrocera cucurbitae; in one the individuals mate early during the day, whereas in the other the individuals mate later. These strains were originally established by divergent artificial selection for developmental time, short and long, with early and late mating times, respectively. The deduced amino acid sequences of PERIOD proteins for these two strains were reported to be identical. Here we cloned another clock gene cryptochrome (cry) from the two strains, and found two stable amino acid substitutions in the strains. In addition, the allele frequency at the two polymorphic sites of cry gene correlated with the circadian locomotor period () across strains, whereas the expression pattern of cry mRNA in the heads of flies taken from the short strain significantly differed from that from the long strain. These findings suggest that variation in the cry gene is related to differences in the circadian behaviour in the two strains, thus implying that the cry gene may have an important role in reproductive isolation.

Original languageEnglish
Pages (from-to)387-392
Number of pages6
JournalHeredity
Volume104
Issue number4
DOIs
Publication statusPublished - Apr 1 2010

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Cryptochromes
Tephritidae
Diptera
Reproductive Isolation
Genes
Cucurbitaceae
Amino Acid Substitution
Gene Frequency
Population
Amino Acid Sequence
Fruit
Head
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Fuchikawa, T., Sanada, S., Nishio, R., Matsumoto, A., Matsuyama, T., Yamagishi, M., ... Miyatake, T. (2010). The clock gene cryptochrome of Bactrocera cucurbitae (Diptera: Tephritidae) in strains with different mating times. Heredity, 104(4), 387-392. https://doi.org/10.1038/hdy.2009.167

The clock gene cryptochrome of Bactrocera cucurbitae (Diptera : Tephritidae) in strains with different mating times. / Fuchikawa, T.; Sanada, S.; Nishio, R.; Matsumoto, A.; Matsuyama, T.; Yamagishi, M.; Tomioka, K.; Tanimura, Teiichi; Miyatake, T.

In: Heredity, Vol. 104, No. 4, 01.04.2010, p. 387-392.

Research output: Contribution to journalArticle

Fuchikawa, T, Sanada, S, Nishio, R, Matsumoto, A, Matsuyama, T, Yamagishi, M, Tomioka, K, Tanimura, T & Miyatake, T 2010, 'The clock gene cryptochrome of Bactrocera cucurbitae (Diptera: Tephritidae) in strains with different mating times', Heredity, vol. 104, no. 4, pp. 387-392. https://doi.org/10.1038/hdy.2009.167
Fuchikawa T, Sanada S, Nishio R, Matsumoto A, Matsuyama T, Yamagishi M et al. The clock gene cryptochrome of Bactrocera cucurbitae (Diptera: Tephritidae) in strains with different mating times. Heredity. 2010 Apr 1;104(4):387-392. https://doi.org/10.1038/hdy.2009.167
Fuchikawa, T. ; Sanada, S. ; Nishio, R. ; Matsumoto, A. ; Matsuyama, T. ; Yamagishi, M. ; Tomioka, K. ; Tanimura, Teiichi ; Miyatake, T. / The clock gene cryptochrome of Bactrocera cucurbitae (Diptera : Tephritidae) in strains with different mating times. In: Heredity. 2010 ; Vol. 104, No. 4. pp. 387-392.
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