The complete amino acid sequence of coagulogen isolated from Southeast Asian horseshoe crab, Carcinoscorpius rotundicauda

Subita Srimal, Toshiyuki Miyata, Shun-Ichiro Kawabata, Takashi Miyata, Sadaaki Iwanaga

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The complete amino acid sequence of coagulogen purified from the hemocytes of the horseshoe crab Carcinoscorpius rotundicauda was determined by characterization of the NH2-terminal sequence and the peptides generated after digestion of the protein with lysyl endopeptidase, Staphylococcal aureus protease V8 and trypsin. Upon sequencing the peptides by the automated Edman method, the following sequence was obtained:1102030A D T N A P L C LC D E P G I L G R NQ L V T P E V K E KIE K A V E A V A EE S G V S G R G F SL F S H H P V F R ECG K Y E C R T V RP E H T R C Y N F PP F V H F T S E C PVS T R D C E P V FG Y T V A G E F R VI V Q A P R A G F RQC V W Q H K C R YG S N N C G F S G RC T Q Q R S V V R LVT Y N L E K D G FL C E S F R T C C GC P C R N YCarcinoscorpius coagulogen consists of a single polypeptide chain with a total of 175 amino acid residues and a calculated molecular weight of 19,675. The secondary structure calculated by the method of Chou and Fasman reveals the presence of an a-helix region in the peptide C segment (residue Nos. 19 to 46), which is released during the proteolytic conversion of coagulogen to coagulin gel. The β-sheet structure and the 16 half-cystines found in the molecule appear to yield a compact protein stable to acid and heat. The amino acid sequences of coagulogen of four species of limulus have been compared and the interspecies evolutionary differences are discussed.

Original languageEnglish
Pages (from-to)305-318
Number of pages14
JournalJournal of biochemistry
Volume98
Issue number2
DOIs
Publication statusPublished - Jan 1 1985

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Horseshoe Crabs
Amino Acid Sequence
Amino Acids
lysyl endopeptidase
Peptides
Dilatation and Curettage
Hemocytes
Thromboplastin
Electrocardiography
Trypsin
Proteolysis
Cysteine
Proteins
Hot Temperature
Molecular Weight
Gels
Molecular weight
Molecules
Acids
coagulogen

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

The complete amino acid sequence of coagulogen isolated from Southeast Asian horseshoe crab, Carcinoscorpius rotundicauda. / Srimal, Subita; Miyata, Toshiyuki; Kawabata, Shun-Ichiro; Miyata, Takashi; Iwanaga, Sadaaki.

In: Journal of biochemistry, Vol. 98, No. 2, 01.01.1985, p. 305-318.

Research output: Contribution to journalArticle

Srimal, Subita ; Miyata, Toshiyuki ; Kawabata, Shun-Ichiro ; Miyata, Takashi ; Iwanaga, Sadaaki. / The complete amino acid sequence of coagulogen isolated from Southeast Asian horseshoe crab, Carcinoscorpius rotundicauda. In: Journal of biochemistry. 1985 ; Vol. 98, No. 2. pp. 305-318.
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