The complete amino acid sequence of ribonuclease from the seeds of bitter gourd (Momordica charantia)

Hiroyuki Ide, Makoto Kimura, Mariko Arai, Gunki Funatsu

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The complete amino acid sequence of ribonuclease (RNase MC) from the seeds of bitter gourd (Momordica charantia) has been determined. This has been achieved by the sequence analysis of peptides derived by enzymatic digestion with trypsin, lysylendopeptidase, and chymotrypsin, as well as by chemical cleavage with cyanogen bromide. The protein contains 191 amino acid residues and has a calculated molecular mass or 21 259 Da. Comparison of this sequence with sequences of the fungal RNases, RNase T2, and RNase Rh, revealed that there are highly conserved residues at positions 32-38 (TXHGLWP) and 81-92 (FWXHEWXKHGTC). Furthermore, the sequence of RNase MC was found to be homologous to those of Nicotiana alata S-glycoproteins involved in self-incompatibility sharing 41% identical residues.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalFEBS Letters
Volume284
Issue number2
DOIs
Publication statusPublished - Jun 24 1991

Fingerprint

Momordica charantia
Ribonucleases
Seed
Amino Acid Sequence
Seeds
Amino Acids
ribonuclease T(2)
Cyanogen Bromide
Protein Sequence Analysis
Molecular mass
Tobacco
Digestion
Glycoproteins
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The complete amino acid sequence of ribonuclease from the seeds of bitter gourd (Momordica charantia). / Ide, Hiroyuki; Kimura, Makoto; Arai, Mariko; Funatsu, Gunki.

In: FEBS Letters, Vol. 284, No. 2, 24.06.1991, p. 161-164.

Research output: Contribution to journalArticle

Ide, Hiroyuki ; Kimura, Makoto ; Arai, Mariko ; Funatsu, Gunki. / The complete amino acid sequence of ribonuclease from the seeds of bitter gourd (Momordica charantia). In: FEBS Letters. 1991 ; Vol. 284, No. 2. pp. 161-164.
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