The conformation formed by the domain after Alanine-155 induces inversion of aspartic acid-151 in αA-crystallin from aged human lenses

Noriko Fujii, Yuko Momose, Miyuki Yamasaki, Tohru Yamagaki, Hiroshi Nakanishi, Toshimasa Uemura, Masatoshi Takita, Noriyuki Ishii

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A new cleavage site, which is a post-translational modification, was found between residues His-154 and Ala-155 in αA-crystallin from the aged human lens. After trypsin digestion of αA-crystallin two peptides that include Asp-151 were obtained and have remarkable differences. That is, the stereo-configuration of the Asp-151 in the normal length peptide was predominately inverted to the D-isomer of β-aspartyl form (D/L of 5.7). However, the stereoconfiguration of the Asp-151 in the cleavage peptide, that lacks the sequence following Ala-155 to the C-terminus, remained predominately in the L-isomer form as indicated by a D/L value of 0.3. The results suggest that the secondary structure in the region of Ala-155 to the C-terminus may constitute a field that causes the inversion of the Asp-151 to the D-isomer form. Since this kind of cleavage was not found in αA-crystallin from young lens, the cleavage between His-154 and Ala-155 is probably the result of aging.

Original languageEnglish
Pages (from-to)918-923
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number3
DOIs
Publication statusPublished - Oct 29 1997

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Crystallins
Aspartic Acid
Isomers
Alanine
Lenses
L Forms
Conformations
Peptides
Post Translational Protein Processing
Trypsin
Digestion
Aging of materials

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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The conformation formed by the domain after Alanine-155 induces inversion of aspartic acid-151 in αA-crystallin from aged human lenses. / Fujii, Noriko; Momose, Yuko; Yamasaki, Miyuki; Yamagaki, Tohru; Nakanishi, Hiroshi; Uemura, Toshimasa; Takita, Masatoshi; Ishii, Noriyuki.

In: Biochemical and Biophysical Research Communications, Vol. 239, No. 3, 29.10.1997, p. 918-923.

Research output: Contribution to journalArticle

Fujii, Noriko ; Momose, Yuko ; Yamasaki, Miyuki ; Yamagaki, Tohru ; Nakanishi, Hiroshi ; Uemura, Toshimasa ; Takita, Masatoshi ; Ishii, Noriyuki. / The conformation formed by the domain after Alanine-155 induces inversion of aspartic acid-151 in αA-crystallin from aged human lenses. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 239, No. 3. pp. 918-923.
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AU - Momose, Yuko

AU - Yamasaki, Miyuki

AU - Yamagaki, Tohru

AU - Nakanishi, Hiroshi

AU - Uemura, Toshimasa

AU - Takita, Masatoshi

AU - Ishii, Noriyuki

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