The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3

Tadashi Hara, Atsushi Terada, Hiroki Yamaguchi, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

Original languageEnglish
Pages (from-to)816-819
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number4
DOIs
Publication statusPublished - May 16 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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