The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization

Atsushi Shimada, Miklós Nyitrai, Ingrid R. Vetter, Dorothee Kühlmann, Beáta Bugyi, Shuh Narumiya, Michael A. Geeves, Alfred Wittinghofer

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Diaphanous-related formins (Drf) are activated by Rho GTP binding proteins and induce polymerization of unbranched actin filaments. They contain three formin homology domains. Evidence as to the effect of formins on actin polymerization were obtained using FH2/FH1 constructs of various length from different Drfs. Here we define the core FH2 domain as a proteolytically stable domain of approximately 338 residues. The monomeric FH2 domains from mDia1 and mDia3 inhibit polymerization of actin and can bind in a 1:1 complex with F-actin at micromolar concentrations. The X-ray structure analysis of the domain shows an elongated, crescent-shaped molecule consisting of three helical subdomains. The most highly conserved regions of the domain span a distance of 75 Å and are both required for barbed-end inhibition. A construct containing an additional 72 residue linker has dramatically different properties: It oligomerizes and induces actin polymerization at subnanomolar concentration.

Original languageEnglish
Pages (from-to)511-522
Number of pages12
JournalMolecular Cell
Volume13
Issue number4
DOIs
Publication statusPublished - Feb 27 2004

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Microfilament Proteins
Polymerization
Actins
rho GTP-Binding Proteins
Actin Cytoskeleton
X-Rays

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization. / Shimada, Atsushi; Nyitrai, Miklós; Vetter, Ingrid R.; Kühlmann, Dorothee; Bugyi, Beáta; Narumiya, Shuh; Geeves, Michael A.; Wittinghofer, Alfred.

In: Molecular Cell, Vol. 13, No. 4, 27.02.2004, p. 511-522.

Research output: Contribution to journalArticle

Shimada, A, Nyitrai, M, Vetter, IR, Kühlmann, D, Bugyi, B, Narumiya, S, Geeves, MA & Wittinghofer, A 2004, 'The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization', Molecular Cell, vol. 13, no. 4, pp. 511-522. https://doi.org/10.1016/S1097-2765(04)00059-0
Shimada, Atsushi ; Nyitrai, Miklós ; Vetter, Ingrid R. ; Kühlmann, Dorothee ; Bugyi, Beáta ; Narumiya, Shuh ; Geeves, Michael A. ; Wittinghofer, Alfred. / The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization. In: Molecular Cell. 2004 ; Vol. 13, No. 4. pp. 511-522.
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