The corneal micropocket assay: A model of angiogenesis and lymphangiogenesis

Shintaro Nakao, Ali Hafezi-Moghadam

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The cornea is a transparent tissue that lacks blood and lymphatic vessels. In addition, the cornea is readily accessible, which makes it convenient for direct visualization of angiogenesis and lymphangiogenesis. The corneal micropocket assay is a commonly used quantitative technique, in which a growth factor containing pellet is micro-surgically implanted into the cornea of a rodent. Subsequently, the growth of the preexisting limbal vessels toward the growth factor is visualized by live microscopy or immunohistochemistry. Recently, there has been signifi cant interest in the process of lymphangiogenesis and the factors that regulate it. To facilitate these studies, we introduce a novel technique for visualization of the immune response during growth factor induced angiogenesis and lymphangiogenesis in the cornea.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages311-316
Number of pages6
DOIs
Publication statusPublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1430
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Nakao, S., & Hafezi-Moghadam, A. (2016). The corneal micropocket assay: A model of angiogenesis and lymphangiogenesis. In Methods in Molecular Biology (pp. 311-316). (Methods in Molecular Biology; Vol. 1430). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3628-1_21