The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1

Maoko Tsukamoto, Kyoko Chiba, Yuriko Sobu, Yuzuha Shiraki, Yuka Okumura, Saori Hata, Akira Kitamura, Tadashi Nakaya, Seiichi Uchida, Masataka Kinjo, Hidenori Taru, Toshiharu Suzuki

Research output: Contribution to journalArticle

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Abstract

Amyloid β-protein precursor (APP) is transported mainly by kinesin-1 and at a higher velocity than other kinesin-1 cargos, such as Alcadein α (Alcα); this is denoted by the enhanced fast velocity (EFV). Interaction of the APP cytoplasmic region with kinesin-1, which is essential for EFV transport, is mediated by JNK-interacting protein 1 (JIP1). To determine the roles of interactions between the APP luminal region and cargo components, we monitored transport of chimeric cargo receptors, Alcα (luminal)–APP (cytoplasmic) and APP (luminal)–Alcα (cytoplasmic). Alcα-APP is transported at the EFV, whereas APP-Alcα is transported at the same velocity as wild-type Alcα. Thus, the cytoplasmic region of APP is necessary and sufficient for the EFV of APP transport by kinesin-1.

Original languageEnglish
Pages (from-to)2716-2724
Number of pages9
JournalFEBS Letters
Volume592
Issue number16
DOIs
Publication statusPublished - Aug 2018

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Kinesin
Amyloid beta-Protein Precursor
Protein Transport

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1. / Tsukamoto, Maoko; Chiba, Kyoko; Sobu, Yuriko; Shiraki, Yuzuha; Okumura, Yuka; Hata, Saori; Kitamura, Akira; Nakaya, Tadashi; Uchida, Seiichi; Kinjo, Masataka; Taru, Hidenori; Suzuki, Toshiharu.

In: FEBS Letters, Vol. 592, No. 16, 08.2018, p. 2716-2724.

Research output: Contribution to journalArticle

Tsukamoto, M, Chiba, K, Sobu, Y, Shiraki, Y, Okumura, Y, Hata, S, Kitamura, A, Nakaya, T, Uchida, S, Kinjo, M, Taru, H & Suzuki, T 2018, 'The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1', FEBS Letters, vol. 592, no. 16, pp. 2716-2724. https://doi.org/10.1002/1873-3468.13204
Tsukamoto, Maoko ; Chiba, Kyoko ; Sobu, Yuriko ; Shiraki, Yuzuha ; Okumura, Yuka ; Hata, Saori ; Kitamura, Akira ; Nakaya, Tadashi ; Uchida, Seiichi ; Kinjo, Masataka ; Taru, Hidenori ; Suzuki, Toshiharu. / The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1. In: FEBS Letters. 2018 ; Vol. 592, No. 16. pp. 2716-2724.
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