The developmental process of xylem embolisms in pine wilt disease monitored by multipoint imaging using compact magnetic resonance imaging

Toshihiro Umebayashi, Kenji Fukuda, Tomoyuki Haishi, Ryo Sotooka, Sule Zuhair, Kyoichi Otsuki

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In pine wilt disease (PWD), embolized tracheids arise after virulent pine wood nematodes (PWN), Bursaphelenchus xylophilus, invade the resin canal of pine tree; infected pine trees finally die from significant loss of xylem water conduction. We used a compact magnetic resonance imaging system with a U-shaped radio frequency (rf) probe coil to reveal the developmental process of the xylem dysfunction in PWD. Multiple cross-sectional slices along the stem axis were acquired to periodically monitor the total water distribution in each 1-year-old main stem of two 3-year-old Japanese black pines (Pinus thunbergii) after inoculation of PWN. During the development of PWD, a mass of embolized tracheids around the inoculation site rapidly enlarged in all directions. This phenomenon occurred before the significant decrease of water potential. Some patch-like embolisms were observed at all monitoring positions during the experimental period. Patchy embolisms in a cross-section did not expand, but the number of patches increased as time passed. When the significant decrease of water potential occurred, the xylem dysfunctional rate near the inoculation point exceeded 70%. Finally, almost the whole area of xylem was abruptly embolized in all cross-sections along the stem. This phenomenon occurred just after water conduction was mostly blocked in one of the cross-sections. Thus, it appears that the simultaneous expansion of embolized conduit clusters may be required to induce a large-scale embolism across the functional xylem. Consequently, xylem dysfunction in infected trees may be closely related to both the distribution and the number of PWN in the pine stem.

Original languageEnglish
Pages (from-to)943-951
Number of pages9
JournalPlant physiology
Volume156
Issue number2
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Xylem
embolism
Embolism
magnetic resonance imaging
xylem
Bursaphelenchus xylophilus
Pinus
Magnetic Resonance Imaging
image analysis
Water
Pinus thunbergii
stems
tracheids
xylem water potential
resin canals
monitoring
water distribution
Radio
water potential
radio

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

The developmental process of xylem embolisms in pine wilt disease monitored by multipoint imaging using compact magnetic resonance imaging. / Umebayashi, Toshihiro; Fukuda, Kenji; Haishi, Tomoyuki; Sotooka, Ryo; Zuhair, Sule; Otsuki, Kyoichi.

In: Plant physiology, Vol. 156, No. 2, 01.06.2011, p. 943-951.

Research output: Contribution to journalArticle

Umebayashi, Toshihiro ; Fukuda, Kenji ; Haishi, Tomoyuki ; Sotooka, Ryo ; Zuhair, Sule ; Otsuki, Kyoichi. / The developmental process of xylem embolisms in pine wilt disease monitored by multipoint imaging using compact magnetic resonance imaging. In: Plant physiology. 2011 ; Vol. 156, No. 2. pp. 943-951.
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