The DJ(H) complex remains active in recombination to V(H) segments after the loss of μ-chain expression in μ-positive pre-B cells

T. Maeda, H. Sugiyama, Y. Tani, S. Kishimoto

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9 Citations (Scopus)

Abstract

AT11-2 is an Abelson virus-transformed B precursor cell line which is capable of differentiating Ig- from μ+ cells via functional recombination of V(H) segments to preexisting DJ(H) complexes. We describe here that after a μ+ subclone (VDJ+/DJ) generated from Ig- AT11-2 (DJ/DJ) cells by in vitro functional V(H) to DJ(H) recombination subsequently lost μ-chain expression either by the recombination of a pseudo V(H) segment to the V(H)DJ(H)+ allele or by the deletion of V(H)DJ(H)+ allele, a novel productive joining of V(H) segments to the preexisting DJ(H) complex occurred. These results indicated that V(H) to V(H)DJ(H) rearrangement was not suppressed in μ-chain producing cells and that the DJ(H) complexes still remained active in the recombination to V(H) segments after the loss of μ-chain expression. Our results may also suggest that V(H) to DJ(H) rearrangement, but not V(H) to V(H)DJ(H) rearrangement, is suppressed in μ-chain producing cells to maintain allelic exclusion. Our cell differentiation system should continue to be valuable for elucidating the mechanism of suppression and associated implications regarding allelic exclusion.

Original languageEnglish
Pages (from-to)3652-3656
Number of pages5
JournalJournal of Immunology
Volume142
Issue number10
Publication statusPublished - Jan 1 1989
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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