The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition

Joel V Tamayo, Takamasa Teramoto, Seema Chatterjee, Traci M Tanaka Hall, Elizabeth R Gavis

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.

Original languageEnglish
Pages (from-to)150-161
Number of pages12
JournalCell Reports
Volume19
Issue number1
DOIs
Publication statusPublished - Apr 4 2017
Externally publishedYes

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Heterogeneous-Nuclear Ribonucleoprotein Group F-H
Drosophila
RNA
Nucleotide Motifs
RNA-Binding Proteins
Molecular Evolution
Alternative Splicing
Protein Biosynthesis
3' Untranslated Regions
Set theory
Crystal structure

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The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition. / Tamayo, Joel V; Teramoto, Takamasa; Chatterjee, Seema; Hall, Traci M Tanaka; Gavis, Elizabeth R.

In: Cell Reports, Vol. 19, No. 1, 04.04.2017, p. 150-161.

Research output: Contribution to journalArticle

Tamayo, Joel V ; Teramoto, Takamasa ; Chatterjee, Seema ; Hall, Traci M Tanaka ; Gavis, Elizabeth R. / The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition. In: Cell Reports. 2017 ; Vol. 19, No. 1. pp. 150-161.
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