TY - JOUR
T1 - The duality of fish gonadotropin receptors
T2 - Cloning and functional characterization of a second gonadotropin receptor cDNA expressed in the ovary and testis of amago salmon (Oncorhynchus rhodurus)
AU - Oba, Yuichi
AU - Hirai, Toshiaki
AU - Yoshiura, Yasutoshi
AU - Yoshikuni, Michiyasu
AU - Kawauchi, Hiroshi
AU - Nagahama, Yoshitaka
N1 - Funding Information:
We thank Dr. C. Morrey for reading the manuscript. This work was supported in part by Grants-in-Aids for Research for the Future (JSPS-RFTF 96L00401) from the Japan Society for the Promotion of Science and for Scientific Research (072831049 and 10440247) from the Japanese Ministry of Education, Science, Sports, and Culture, Japan, and by a Grant-in-Aid (Bio Design Program) from the Ministry of Agriculture, Forestry, and Fisheries, Japan.
PY - 1999/11/19
Y1 - 1999/11/19
N2 - We previously isolated a cDNA encoding a gonadotropin receptor (sGTH-R) from amago salmon (Oncorhynchus rhodurus) ovarian follicles. In the present study, we cloned a second gonadotropin receptor (sGTH-RI) from the same RNA preparations. Overall sequence homology between sGTH-RI and sGTH-R is 44%. The highest homology occurs with mammalian FSH receptors (49%). Functional characterization examined in COS-7 cells transiently transfected with sGTH-RI showed the largest increase in cAMP production when exposed to salmon GTH I. These results provide the first evidence in any ectothermic vertebrate, the amago salmon, of the duality of gonadotropin receptors.
AB - We previously isolated a cDNA encoding a gonadotropin receptor (sGTH-R) from amago salmon (Oncorhynchus rhodurus) ovarian follicles. In the present study, we cloned a second gonadotropin receptor (sGTH-RI) from the same RNA preparations. Overall sequence homology between sGTH-RI and sGTH-R is 44%. The highest homology occurs with mammalian FSH receptors (49%). Functional characterization examined in COS-7 cells transiently transfected with sGTH-RI showed the largest increase in cAMP production when exposed to salmon GTH I. These results provide the first evidence in any ectothermic vertebrate, the amago salmon, of the duality of gonadotropin receptors.
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U2 - 10.1006/bbrc.1999.1700
DO - 10.1006/bbrc.1999.1700
M3 - Article
C2 - 10558873
AN - SCOPUS:0033584974
SN - 0006-291X
VL - 265
SP - 366
EP - 371
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -