The duality of fish gonadotropin receptors: Cloning and functional characterization of a second gonadotropin receptor cDNA expressed in the ovary and testis of amago salmon (Oncorhynchus rhodurus)

Yuichi Oba, Toshiaki Hirai, Yasutoshi Yoshiura, Michiyasu Yoshikuni, Hiroshi Kawauchi, Yoshitaka Nagahama

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

We previously isolated a cDNA encoding a gonadotropin receptor (sGTH-R) from amago salmon (Oncorhynchus rhodurus) ovarian follicles. In the present study, we cloned a second gonadotropin receptor (sGTH-RI) from the same RNA preparations. Overall sequence homology between sGTH-RI and sGTH-R is 44%. The highest homology occurs with mammalian FSH receptors (49%). Functional characterization examined in COS-7 cells transiently transfected with sGTH-RI showed the largest increase in cAMP production when exposed to salmon GTH I. These results provide the first evidence in any ectothermic vertebrate, the amago salmon, of the duality of gonadotropin receptors.

Original languageEnglish
Pages (from-to)366-371
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume265
Issue number2
DOIs
Publication statusPublished - Nov 19 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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