The dynamin-related protein Vps1 regulates vacuole fission, fusion and tubulation in the fission yeast, Schizosaccharomyces pombe

Sarah Röthlisberger, Isabelle Jourdain, Chad Johnson, Kaoru Takegawa, Jeremy S. Hyams

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fission yeast cells lacking the dynamin-related protein (DRP) Vps1 had smaller vacuoles with reduced capacity for both fusion and fission in response to hypotonic and hypertonic conditions respectively. vps1Δ cells showed normal vacuolar protein sorting, actin organisation and endocytosis. Over-expression of vps1 transformed vacuoles from spherical to tubular. Tubule formation was enhanced in fission conditions and required the Rab protein Ypt7. Vacuole tubulation by Vps1 was more extensive in the absence of a second DRP, Dnm1. Both dnm1Δ and the double mutant vps1Δ dnm1Δ showed vacuole fission defects similar to that of vps1Δ. Over-expression of vps1 in dnm1Δ, or of dnm1 in vps1Δ failed to rescue this phenotype. Over-expression of dnm1 in wild-type cells, on the other hand, induced vacuole fission. Our results are consistent with a model of vacuole fission in which Vps1 creates a tubule of an appropriate diameter for subsequent scission by Dnm1.

Original languageEnglish
Pages (from-to)927-935
Number of pages9
JournalFungal Genetics and Biology
Volume46
Issue number12
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Fingerprint

Dynamins
Schizosaccharomyces
Vacuoles
Proteins
Protein Transport
Endocytosis
Actins
Phenotype

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

The dynamin-related protein Vps1 regulates vacuole fission, fusion and tubulation in the fission yeast, Schizosaccharomyces pombe. / Röthlisberger, Sarah; Jourdain, Isabelle; Johnson, Chad; Takegawa, Kaoru; Hyams, Jeremy S.

In: Fungal Genetics and Biology, Vol. 46, No. 12, 01.12.2009, p. 927-935.

Research output: Contribution to journalArticle

Röthlisberger, Sarah ; Jourdain, Isabelle ; Johnson, Chad ; Takegawa, Kaoru ; Hyams, Jeremy S. / The dynamin-related protein Vps1 regulates vacuole fission, fusion and tubulation in the fission yeast, Schizosaccharomyces pombe. In: Fungal Genetics and Biology. 2009 ; Vol. 46, No. 12. pp. 927-935.
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