The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects

T. Handa, Takahisa Anada, Y. Honda, H. Yamazaki, K. Kobayashi, N. Kanda, S. Kamakura, S. Echigo, O. Suzuki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

This study was designed to investigate the extent to which an octacalcium phosphate/gelatin (OCP/Gel) composite can repair rat calvarial critical-sized defects (CSD). OCP crystals were grown with various concentrations of gelatin molecules and the OCP/Gel composites were characterized by chemical analysis, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), selected area electron diffraction (SAED) and mercury intrusion porosimetry. The OCP/Gel composite disks received vacuum dehydrothermal treatment, were implanted in Wistar rat calvarial CSD for 4, 8 and 16 weeks, and then subjected to radiologic, histologic, histomorphometric and histochemical assessment. The attachment of mouse bone marrow stromal ST-2 cells on the disks of the OCP/Gel composites was also examined after 1 day of incubation. OCP/Gel composites containing 24 wt.%, 31 wt.% and 40 wt.% of OCP and with approximate pore sizes of 10-500 μm were obtained. Plate-like crystals were observed closely associated with the Gel matrices. TEM, XRD, FTIR and SAED confirmed that the plate-like crystals were identical to those of the OCP phase, but contained a small amount of sphere-like amorphous material adjacent to the OCP crystals. The OCP (40 wt.%)/Gel composite repaired 71% of the CSD in conjunction with material degradation by osteoclastic cells, which reduced the percentage of the remaining implant to less than 3% within 16 weeks. Of the seeded ST-2 cells, 60-70% were able to migrate and attach to the OCP/Gel composites after 1 day of incubation, regardless of the OCP content. These results indicate that an OCP/Gel composite can repair rat calvarial CSD very efficiently and has favorable biodegradation characteristics. Therefore, it is hypothesized that host osteoblastic cells can easily migrate into an OCP/Gel composite.

Original languageEnglish
Pages (from-to)1190-1200
Number of pages11
JournalActa Biomaterialia
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 1 2012
Externally publishedYes

Fingerprint

Gelatin
Rats
Phosphates
Repair
Defects
Composite materials
Crystals
Transmission Electron Microscopy
Electron diffraction
X-Ray Diffraction
Gels
Electrons
Transmission electron microscopy
octacalcium phosphate
X ray diffraction
Mercury (metal)
Fourier Analysis
Fourier Transform Infrared Spectroscopy
Vacuum
Biodegradation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects. / Handa, T.; Anada, Takahisa; Honda, Y.; Yamazaki, H.; Kobayashi, K.; Kanda, N.; Kamakura, S.; Echigo, S.; Suzuki, O.

In: Acta Biomaterialia, Vol. 8, No. 3, 01.03.2012, p. 1190-1200.

Research output: Contribution to journalArticle

Handa, T, Anada, T, Honda, Y, Yamazaki, H, Kobayashi, K, Kanda, N, Kamakura, S, Echigo, S & Suzuki, O 2012, 'The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects', Acta Biomaterialia, vol. 8, no. 3, pp. 1190-1200. https://doi.org/10.1016/j.actbio.2011.12.002
Handa, T. ; Anada, Takahisa ; Honda, Y. ; Yamazaki, H. ; Kobayashi, K. ; Kanda, N. ; Kamakura, S. ; Echigo, S. ; Suzuki, O. / The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects. In: Acta Biomaterialia. 2012 ; Vol. 8, No. 3. pp. 1190-1200.
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AU - Kobayashi, K.

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