The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes

Douglas R. Drummond, John Armstrong, Alan Colman

Research output: Contribution to journalArticlepeer-review

133 Citations (Scopus)

Abstract

Synthetic RNAs coding for chicken lysozyme, calf preprochymozin and Xenopus globin were transcribed in vitro using Sp6 RNA polymerase. The effects of capping and adding a poly(A) tail on the stability, movement and translation of these RNAs in Xenopus oocytes was examined.Capping and polyadenylation increased stability of the transcripts, with at least 40% remaining intact 48h after injection into oocytes.Capped poly(A) transcripts moved more rapidly in oocytes than either capped poly(A)+ transcripts or naturally occurring mRNAs.The translational efficiency of most of the synthetic RNAs in oocytes increased with both capping and polyadenylation. The exception was one Xenopus globin transcript which had an unusual 3′ end of 20As and 30Cs, where further polyadenylation decreased translational efficiency.Polyadenylation was essential for detectable expression of the synthetic RNAs in cultured cells, but decreased translation of the synthetic RNAs in vitro.

Original languageEnglish
Pages (from-to)7375-7394
Number of pages20
JournalNucleic acids research
Volume13
Issue number20
DOIs
Publication statusPublished - Oct 25 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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