The effect of cyclin D1 overexpression in human head and neck cancer cells

Torahiko Nakashima, Yuichiro Kuratomi, Ryuji Yasumatsu, Muneyuki Masuda, Koji Koike, Toshiro Umezaki, Gary L. Clayman, Takashi Nakagawa, Shizuo Komune

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7 Citations (Scopus)

Abstract

Overexpression of cyclin D1 in head and neck cancer has been suggested to be a poor prognostic factor. To understand the role of cyclin D1 expression in head and neck cancer, we overexpressed cyclin D1 in TU182 (a cell line derived from pharyngeal cancer) using a retroviral vector. Stable transfectants were isolated by neomycin (G418) selection. Compared to the parental and control-vector transfected cells, the cyclin D1 transfected cells revealed a decrease of the G1/G0 population and resulted in continuous proliferation under low serum conditions. Proliferation assays revealed an increase in resistance to cisplatin in cyclin D1 overexpressing cells. These observation suggest that deregulation of cyclin D1 may reduce growth factor requirements and contribute to the resistance to some chemotherapeutic agents among head and neck cancer patients.

Original languageEnglish
Pages (from-to)379-383
Number of pages5
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume262
Issue number5
DOIs
Publication statusPublished - May 1 2005

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All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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