The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses

D. A. Martin, K. Nonaka, K. I. Yanagita, M. Nakata

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The embryo transfer technique and cephalometry were used to investigate the effect of dam strain on intrauterine craniofacial growth and the severity of cleft lip and palate (CLP) in a CLP-susceptible CL/Fr strain of embryos. The CL/Fr strain of embryos at early blastocyst stage was transferred to the same dam strain and to the CLP-resistant C57BL dam strain. On the 18th gestational day, each dam was laparotomized to take out the fetuses. The spontaneous incidence of CLP in the fetuses was checked and a cephalometric observation of the craniofacial complex of each fetus was done just after laparotomy. The dorsoventral craniofacial size of the unaffected fetuses and the severity of CLP in the affected ones were compared between both dam strains. The following results were obtained: 1) The overall craniofacial sizes of the unaffected fetuses observed in the CL/Fr dam strain were significantly smaller than those seen in the C57BL dam strain. Those of the affected fetuses observed in the CL/Fr dam strain were smaller than those seen in the C57BL dam strain, although the interstrain difference was not significant. 2) The dam strain had a highly significant effect on the craniofacial size of the unaffected fetuses. 3) The CLP frequency in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. 4) The severity of CLP in the affected fetuses observed in the CL/Fr dam strain was significantly more serious than that seen in the C57BL dam strain. These results indicated that the CLP-susceptible CL/Fr dam strain retarded the intrauterine craniofacial growth of the fetuses and that the cleft condition in the affected fetuses observed in the CL/Fr dam strain was more seriously affected than that seen in the CLP-resistant C57BL dam strain. Thus, it can be concluded that the effect of the dam strain played an important role in the craniofacial morphogenesis of the CL/Fr strain of mouse fetuses that developed from the embryo transferred to the CL/Fr and C57BL dam strains along with the genotype of embryos.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalJournal of Craniofacial Genetics and Developmental Biology
Volume15
Issue number3
Publication statusPublished - Jan 1 1995

Fingerprint

Morphogenesis
Cleft Lip
Cleft Palate
Fetus
Embryonic Structures
Cephalometry
Embryo Transfer
Blastocyst
Growth
Laparotomy
Genotype
Observation
Incidence

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses. / Martin, D. A.; Nonaka, K.; Yanagita, K. I.; Nakata, M.

In: Journal of Craniofacial Genetics and Developmental Biology, Vol. 15, No. 3, 01.01.1995, p. 117-124.

Research output: Contribution to journalArticle

Martin, D. A. ; Nonaka, K. ; Yanagita, K. I. ; Nakata, M. / The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses. In: Journal of Craniofacial Genetics and Developmental Biology. 1995 ; Vol. 15, No. 3. pp. 117-124.
@article{1c25355f52d7426ab779e6c6ba90ffbc,
title = "The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses",
abstract = "The embryo transfer technique and cephalometry were used to investigate the effect of dam strain on intrauterine craniofacial growth and the severity of cleft lip and palate (CLP) in a CLP-susceptible CL/Fr strain of embryos. The CL/Fr strain of embryos at early blastocyst stage was transferred to the same dam strain and to the CLP-resistant C57BL dam strain. On the 18th gestational day, each dam was laparotomized to take out the fetuses. The spontaneous incidence of CLP in the fetuses was checked and a cephalometric observation of the craniofacial complex of each fetus was done just after laparotomy. The dorsoventral craniofacial size of the unaffected fetuses and the severity of CLP in the affected ones were compared between both dam strains. The following results were obtained: 1) The overall craniofacial sizes of the unaffected fetuses observed in the CL/Fr dam strain were significantly smaller than those seen in the C57BL dam strain. Those of the affected fetuses observed in the CL/Fr dam strain were smaller than those seen in the C57BL dam strain, although the interstrain difference was not significant. 2) The dam strain had a highly significant effect on the craniofacial size of the unaffected fetuses. 3) The CLP frequency in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. 4) The severity of CLP in the affected fetuses observed in the CL/Fr dam strain was significantly more serious than that seen in the C57BL dam strain. These results indicated that the CLP-susceptible CL/Fr dam strain retarded the intrauterine craniofacial growth of the fetuses and that the cleft condition in the affected fetuses observed in the CL/Fr dam strain was more seriously affected than that seen in the CLP-resistant C57BL dam strain. Thus, it can be concluded that the effect of the dam strain played an important role in the craniofacial morphogenesis of the CL/Fr strain of mouse fetuses that developed from the embryo transferred to the CL/Fr and C57BL dam strains along with the genotype of embryos.",
author = "Martin, {D. A.} and K. Nonaka and Yanagita, {K. I.} and M. Nakata",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "15",
pages = "117--124",
journal = "Journal of Craniofacial Genetics and Developmental Biology",
issn = "0270-4145",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses

AU - Martin, D. A.

AU - Nonaka, K.

AU - Yanagita, K. I.

AU - Nakata, M.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The embryo transfer technique and cephalometry were used to investigate the effect of dam strain on intrauterine craniofacial growth and the severity of cleft lip and palate (CLP) in a CLP-susceptible CL/Fr strain of embryos. The CL/Fr strain of embryos at early blastocyst stage was transferred to the same dam strain and to the CLP-resistant C57BL dam strain. On the 18th gestational day, each dam was laparotomized to take out the fetuses. The spontaneous incidence of CLP in the fetuses was checked and a cephalometric observation of the craniofacial complex of each fetus was done just after laparotomy. The dorsoventral craniofacial size of the unaffected fetuses and the severity of CLP in the affected ones were compared between both dam strains. The following results were obtained: 1) The overall craniofacial sizes of the unaffected fetuses observed in the CL/Fr dam strain were significantly smaller than those seen in the C57BL dam strain. Those of the affected fetuses observed in the CL/Fr dam strain were smaller than those seen in the C57BL dam strain, although the interstrain difference was not significant. 2) The dam strain had a highly significant effect on the craniofacial size of the unaffected fetuses. 3) The CLP frequency in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. 4) The severity of CLP in the affected fetuses observed in the CL/Fr dam strain was significantly more serious than that seen in the C57BL dam strain. These results indicated that the CLP-susceptible CL/Fr dam strain retarded the intrauterine craniofacial growth of the fetuses and that the cleft condition in the affected fetuses observed in the CL/Fr dam strain was more seriously affected than that seen in the CLP-resistant C57BL dam strain. Thus, it can be concluded that the effect of the dam strain played an important role in the craniofacial morphogenesis of the CL/Fr strain of mouse fetuses that developed from the embryo transferred to the CL/Fr and C57BL dam strains along with the genotype of embryos.

AB - The embryo transfer technique and cephalometry were used to investigate the effect of dam strain on intrauterine craniofacial growth and the severity of cleft lip and palate (CLP) in a CLP-susceptible CL/Fr strain of embryos. The CL/Fr strain of embryos at early blastocyst stage was transferred to the same dam strain and to the CLP-resistant C57BL dam strain. On the 18th gestational day, each dam was laparotomized to take out the fetuses. The spontaneous incidence of CLP in the fetuses was checked and a cephalometric observation of the craniofacial complex of each fetus was done just after laparotomy. The dorsoventral craniofacial size of the unaffected fetuses and the severity of CLP in the affected ones were compared between both dam strains. The following results were obtained: 1) The overall craniofacial sizes of the unaffected fetuses observed in the CL/Fr dam strain were significantly smaller than those seen in the C57BL dam strain. Those of the affected fetuses observed in the CL/Fr dam strain were smaller than those seen in the C57BL dam strain, although the interstrain difference was not significant. 2) The dam strain had a highly significant effect on the craniofacial size of the unaffected fetuses. 3) The CLP frequency in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. 4) The severity of CLP in the affected fetuses observed in the CL/Fr dam strain was significantly more serious than that seen in the C57BL dam strain. These results indicated that the CLP-susceptible CL/Fr dam strain retarded the intrauterine craniofacial growth of the fetuses and that the cleft condition in the affected fetuses observed in the CL/Fr dam strain was more seriously affected than that seen in the CLP-resistant C57BL dam strain. Thus, it can be concluded that the effect of the dam strain played an important role in the craniofacial morphogenesis of the CL/Fr strain of mouse fetuses that developed from the embryo transferred to the CL/Fr and C57BL dam strains along with the genotype of embryos.

UR - http://www.scopus.com/inward/record.url?scp=0029150248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029150248&partnerID=8YFLogxK

M3 - Article

C2 - 8642051

AN - SCOPUS:0029150248

VL - 15

SP - 117

EP - 124

JO - Journal of Craniofacial Genetics and Developmental Biology

JF - Journal of Craniofacial Genetics and Developmental Biology

SN - 0270-4145

IS - 3

ER -