The effect of fetal stabilization using morphine hydrochloride on neonatal rats

Toru Uesugi, Kouji Masumoto, Kouji Nagata, Tomoaki Taguchi, Sachiyo Suita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously showed that fetal stabilization (FS) could improve the prognosis of congenital diaphragmatic hernia (CDH) patients. The aim of this study is to elucidate the effect of FS in normal neonatal rats. Pregnant Sprague-Dawley rats were treated by experimental protocols on day 21 of gestation. In the FS-group, they received morphine hydrochloride via the placenta before undergoing a caesarean section. In the control group (C-group), they received no morphine hydrochloride. All neonatal rats were managed under mechanical ventilation. We collected the blood samples and bronchoalveolar lavage fluid (BALF) at birth and at 4 h after birth in both groups and the cytokine levels in those samples were measured. The specimens obtained from the right lung were stained with anti-TNF-α antibody. The levels of serum TNF-α at birth and IL-6 at 4 h after birth in the FS-group decreased, in comparison to those in the C-group. The staining intensity of anti-TNF-α antibody in the FS-group was weaker than that in the C-group. FS reduced the production of inflammatory cytokines on neonatal rats, which was controlled by mechanical ventilation. This effect may beneficially reduce the occurrence of persistent pulmonary hypertension of neonate (PPHN), which is induced by stress in CDH patients.

Original languageEnglish
Pages (from-to)961-965
Number of pages5
JournalPediatric surgery international
Volume23
Issue number10
DOIs
Publication statusPublished - Oct 1 2007

Fingerprint

Morphine
Parturition
Artificial Respiration
Control Groups
Anti-Idiotypic Antibodies
Cytokines
Bronchoalveolar Lavage Fluid
Pulmonary Hypertension
Cesarean Section
Placenta
Sprague Dawley Rats
Interleukin-6
Newborn Infant
Staining and Labeling
Pregnancy
Lung
Serum
Congenital Diaphragmatic Hernias

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Surgery

Cite this

The effect of fetal stabilization using morphine hydrochloride on neonatal rats. / Uesugi, Toru; Masumoto, Kouji; Nagata, Kouji; Taguchi, Tomoaki; Suita, Sachiyo.

In: Pediatric surgery international, Vol. 23, No. 10, 01.10.2007, p. 961-965.

Research output: Contribution to journalArticle

Uesugi, Toru ; Masumoto, Kouji ; Nagata, Kouji ; Taguchi, Tomoaki ; Suita, Sachiyo. / The effect of fetal stabilization using morphine hydrochloride on neonatal rats. In: Pediatric surgery international. 2007 ; Vol. 23, No. 10. pp. 961-965.
@article{e9e2565fb8d542a49728445c008d66f8,
title = "The effect of fetal stabilization using morphine hydrochloride on neonatal rats",
abstract = "We previously showed that fetal stabilization (FS) could improve the prognosis of congenital diaphragmatic hernia (CDH) patients. The aim of this study is to elucidate the effect of FS in normal neonatal rats. Pregnant Sprague-Dawley rats were treated by experimental protocols on day 21 of gestation. In the FS-group, they received morphine hydrochloride via the placenta before undergoing a caesarean section. In the control group (C-group), they received no morphine hydrochloride. All neonatal rats were managed under mechanical ventilation. We collected the blood samples and bronchoalveolar lavage fluid (BALF) at birth and at 4 h after birth in both groups and the cytokine levels in those samples were measured. The specimens obtained from the right lung were stained with anti-TNF-α antibody. The levels of serum TNF-α at birth and IL-6 at 4 h after birth in the FS-group decreased, in comparison to those in the C-group. The staining intensity of anti-TNF-α antibody in the FS-group was weaker than that in the C-group. FS reduced the production of inflammatory cytokines on neonatal rats, which was controlled by mechanical ventilation. This effect may beneficially reduce the occurrence of persistent pulmonary hypertension of neonate (PPHN), which is induced by stress in CDH patients.",
author = "Toru Uesugi and Kouji Masumoto and Kouji Nagata and Tomoaki Taguchi and Sachiyo Suita",
year = "2007",
month = "10",
day = "1",
doi = "10.1007/s00383-007-1978-7",
language = "English",
volume = "23",
pages = "961--965",
journal = "Pediatric Surgery International",
issn = "0179-0358",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - The effect of fetal stabilization using morphine hydrochloride on neonatal rats

AU - Uesugi, Toru

AU - Masumoto, Kouji

AU - Nagata, Kouji

AU - Taguchi, Tomoaki

AU - Suita, Sachiyo

PY - 2007/10/1

Y1 - 2007/10/1

N2 - We previously showed that fetal stabilization (FS) could improve the prognosis of congenital diaphragmatic hernia (CDH) patients. The aim of this study is to elucidate the effect of FS in normal neonatal rats. Pregnant Sprague-Dawley rats were treated by experimental protocols on day 21 of gestation. In the FS-group, they received morphine hydrochloride via the placenta before undergoing a caesarean section. In the control group (C-group), they received no morphine hydrochloride. All neonatal rats were managed under mechanical ventilation. We collected the blood samples and bronchoalveolar lavage fluid (BALF) at birth and at 4 h after birth in both groups and the cytokine levels in those samples were measured. The specimens obtained from the right lung were stained with anti-TNF-α antibody. The levels of serum TNF-α at birth and IL-6 at 4 h after birth in the FS-group decreased, in comparison to those in the C-group. The staining intensity of anti-TNF-α antibody in the FS-group was weaker than that in the C-group. FS reduced the production of inflammatory cytokines on neonatal rats, which was controlled by mechanical ventilation. This effect may beneficially reduce the occurrence of persistent pulmonary hypertension of neonate (PPHN), which is induced by stress in CDH patients.

AB - We previously showed that fetal stabilization (FS) could improve the prognosis of congenital diaphragmatic hernia (CDH) patients. The aim of this study is to elucidate the effect of FS in normal neonatal rats. Pregnant Sprague-Dawley rats were treated by experimental protocols on day 21 of gestation. In the FS-group, they received morphine hydrochloride via the placenta before undergoing a caesarean section. In the control group (C-group), they received no morphine hydrochloride. All neonatal rats were managed under mechanical ventilation. We collected the blood samples and bronchoalveolar lavage fluid (BALF) at birth and at 4 h after birth in both groups and the cytokine levels in those samples were measured. The specimens obtained from the right lung were stained with anti-TNF-α antibody. The levels of serum TNF-α at birth and IL-6 at 4 h after birth in the FS-group decreased, in comparison to those in the C-group. The staining intensity of anti-TNF-α antibody in the FS-group was weaker than that in the C-group. FS reduced the production of inflammatory cytokines on neonatal rats, which was controlled by mechanical ventilation. This effect may beneficially reduce the occurrence of persistent pulmonary hypertension of neonate (PPHN), which is induced by stress in CDH patients.

UR - http://www.scopus.com/inward/record.url?scp=34548795501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548795501&partnerID=8YFLogxK

U2 - 10.1007/s00383-007-1978-7

DO - 10.1007/s00383-007-1978-7

M3 - Article

C2 - 17653557

AN - SCOPUS:34548795501

VL - 23

SP - 961

EP - 965

JO - Pediatric Surgery International

JF - Pediatric Surgery International

SN - 0179-0358

IS - 10

ER -