The effect of heavy metals on nicotinamide N-methyltransferase activity in vitro relating to Parkinson's disease

Akiko Sugawara, Hiroko Yokoyama, Mitsuhiro Ohta, Takafumi Maeda, Kazuko Tanaka, Tetsuhito Fukushima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: The aims of this study were to determine the effects of heavy metals such as manganese on nicotinamide N-methyltransferase (EC 2.1.1.1) (NNMT) activity and to consider the possibility of involvement of NNMT activation in the pathogenesis of heavy metal induced Parkinson's disease. Methods: NNMT activity in supernatants separated from brain, liver and kidney homogenates of 5 elderly male Wistar rats by centrifugation were measured by high performance liquid chromatography system with fluorescence. NNMT activity under the conditon of 0.5 or 5.0 mM Mn2+, Fe2+, Cu2+ or Cd 2+ was compared with control (no metal ion existence). Results: NNMT activities in rat brain, liver and kidneys were significantly decreased by Cu2+, and those in the liver and kidneys were significantly decreased by Cd2+. Mn2+ reduced NNMT activity only in the liver. Fe2+ had no effect on NNMT activity. Conclusions: No metal increased NNMT activity in this study, contrary to our hypothesis. Further study is needed to clarify the reason why the effects of Mn2+ and Fe2+ which have a high relevance to Parkinson's disease on NNMT activity differ from those of Cu2+ and Cd2+.

Original languageEnglish
Pages (from-to)180-183
Number of pages4
JournalEnvironmental health and preventive medicine
Volume10
Issue number4
DOIs
Publication statusPublished - Jul 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

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