The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting

Akira Ito, Kousuke Ino, Takeshi Kobayashi, Hiroyuki Honda

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Tissue engineering requires novel technologies for establishing 3D constructs, and the layered method of culturing cell sheets (cell sheet engineering) is one potentially useful approach. In the present study, we investigated whether coating the culture surface with RGD (Arg-Gly-Asp) peptide-conjugated magnetite cationic liposomes (RGD-MCLs) was able to facilitate cell growth, cell sheet construction and cell sheet harvest using magnetic force without enzymatic treatment. To promote cell attachment, an RGD-motif-containing peptide was coupled to the phospholipid of our original magnetite cationic liposomes (MCLs). The RGD-MCLs were added to a commercially available 24-well ultra-low-attachment plate the surface of which comprised a covalently bound hydrogel layer that was hydrophilic and neutrally charged. A magnet was placed on the underside of the well in order to attract the RGD-MCLs to the surface of the well, and then NIH/3T3 cells were seeded into the well. Cells adhered to the bottom of the culture surface, which was coated with RGD-MCLs, and the cells spread and proliferated to confluency. After incubation, the magnet was removed and the cells were detached from the bottom of the plates, forming a contiguous cell sheet. Because the sheets contained magnetite nanoparticles, they could be harvested using a magnet inserted into the well. These results suggest that this novel methodology using RGD-MCLs and magnetic force, which we have termed 'magnetic force-based tissue engineering (Mag-TE)', is a promising approach for tissue engineering.

Original languageEnglish
Pages (from-to)6185-6193
Number of pages9
JournalBiomaterials
Volume26
Issue number31
DOIs
Publication statusPublished - Nov 1 2005

Fingerprint

Ferrosoferric Oxide
Liposomes
Cell growth
Magnetite
Peptides
Growth
Tissue engineering
Magnets
Tissue Engineering
Magnetite Nanoparticles
Magnetite nanoparticles
Hydrogel
Phospholipids
Hydrogels
Cell Engineering
arginyl-glycyl-aspartic acid
NIH 3T3 Cells
Coatings
Technology

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting. / Ito, Akira; Ino, Kousuke; Kobayashi, Takeshi; Honda, Hiroyuki.

In: Biomaterials, Vol. 26, No. 31, 01.11.2005, p. 6185-6193.

Research output: Contribution to journalArticle

Ito, Akira ; Ino, Kousuke ; Kobayashi, Takeshi ; Honda, Hiroyuki. / The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting. In: Biomaterials. 2005 ; Vol. 26, No. 31. pp. 6185-6193.
@article{a592f1c9846d42f08b908b33ae1d1f3e,
title = "The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting",
abstract = "Tissue engineering requires novel technologies for establishing 3D constructs, and the layered method of culturing cell sheets (cell sheet engineering) is one potentially useful approach. In the present study, we investigated whether coating the culture surface with RGD (Arg-Gly-Asp) peptide-conjugated magnetite cationic liposomes (RGD-MCLs) was able to facilitate cell growth, cell sheet construction and cell sheet harvest using magnetic force without enzymatic treatment. To promote cell attachment, an RGD-motif-containing peptide was coupled to the phospholipid of our original magnetite cationic liposomes (MCLs). The RGD-MCLs were added to a commercially available 24-well ultra-low-attachment plate the surface of which comprised a covalently bound hydrogel layer that was hydrophilic and neutrally charged. A magnet was placed on the underside of the well in order to attract the RGD-MCLs to the surface of the well, and then NIH/3T3 cells were seeded into the well. Cells adhered to the bottom of the culture surface, which was coated with RGD-MCLs, and the cells spread and proliferated to confluency. After incubation, the magnet was removed and the cells were detached from the bottom of the plates, forming a contiguous cell sheet. Because the sheets contained magnetite nanoparticles, they could be harvested using a magnet inserted into the well. These results suggest that this novel methodology using RGD-MCLs and magnetic force, which we have termed 'magnetic force-based tissue engineering (Mag-TE)', is a promising approach for tissue engineering.",
author = "Akira Ito and Kousuke Ino and Takeshi Kobayashi and Hiroyuki Honda",
year = "2005",
month = "11",
day = "1",
doi = "10.1016/j.biomaterials.2005.03.039",
language = "English",
volume = "26",
pages = "6185--6193",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "31",

}

TY - JOUR

T1 - The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting

AU - Ito, Akira

AU - Ino, Kousuke

AU - Kobayashi, Takeshi

AU - Honda, Hiroyuki

PY - 2005/11/1

Y1 - 2005/11/1

N2 - Tissue engineering requires novel technologies for establishing 3D constructs, and the layered method of culturing cell sheets (cell sheet engineering) is one potentially useful approach. In the present study, we investigated whether coating the culture surface with RGD (Arg-Gly-Asp) peptide-conjugated magnetite cationic liposomes (RGD-MCLs) was able to facilitate cell growth, cell sheet construction and cell sheet harvest using magnetic force without enzymatic treatment. To promote cell attachment, an RGD-motif-containing peptide was coupled to the phospholipid of our original magnetite cationic liposomes (MCLs). The RGD-MCLs were added to a commercially available 24-well ultra-low-attachment plate the surface of which comprised a covalently bound hydrogel layer that was hydrophilic and neutrally charged. A magnet was placed on the underside of the well in order to attract the RGD-MCLs to the surface of the well, and then NIH/3T3 cells were seeded into the well. Cells adhered to the bottom of the culture surface, which was coated with RGD-MCLs, and the cells spread and proliferated to confluency. After incubation, the magnet was removed and the cells were detached from the bottom of the plates, forming a contiguous cell sheet. Because the sheets contained magnetite nanoparticles, they could be harvested using a magnet inserted into the well. These results suggest that this novel methodology using RGD-MCLs and magnetic force, which we have termed 'magnetic force-based tissue engineering (Mag-TE)', is a promising approach for tissue engineering.

AB - Tissue engineering requires novel technologies for establishing 3D constructs, and the layered method of culturing cell sheets (cell sheet engineering) is one potentially useful approach. In the present study, we investigated whether coating the culture surface with RGD (Arg-Gly-Asp) peptide-conjugated magnetite cationic liposomes (RGD-MCLs) was able to facilitate cell growth, cell sheet construction and cell sheet harvest using magnetic force without enzymatic treatment. To promote cell attachment, an RGD-motif-containing peptide was coupled to the phospholipid of our original magnetite cationic liposomes (MCLs). The RGD-MCLs were added to a commercially available 24-well ultra-low-attachment plate the surface of which comprised a covalently bound hydrogel layer that was hydrophilic and neutrally charged. A magnet was placed on the underside of the well in order to attract the RGD-MCLs to the surface of the well, and then NIH/3T3 cells were seeded into the well. Cells adhered to the bottom of the culture surface, which was coated with RGD-MCLs, and the cells spread and proliferated to confluency. After incubation, the magnet was removed and the cells were detached from the bottom of the plates, forming a contiguous cell sheet. Because the sheets contained magnetite nanoparticles, they could be harvested using a magnet inserted into the well. These results suggest that this novel methodology using RGD-MCLs and magnetic force, which we have termed 'magnetic force-based tissue engineering (Mag-TE)', is a promising approach for tissue engineering.

UR - http://www.scopus.com/inward/record.url?scp=20444474657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444474657&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2005.03.039

DO - 10.1016/j.biomaterials.2005.03.039

M3 - Article

C2 - 15899515

AN - SCOPUS:20444474657

VL - 26

SP - 6185

EP - 6193

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 31

ER -