The effect of saliva or serum on bacterial and Candida albicans colonization on type I collagen

H. Nikawa, H. Egusa, H. Yamashiro, M. Nishimura, S. Makihira, C. Jin, H. Fukushima, T. Hamada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Colonization of Candida albicans on oral surfaces can serve as a reservoir for disseminated infections, such as aspiration pneumonia and gastrointestinal infection, particularly in the immuno-compromised host. Therefore, the aim of this study was to investigate the effects of salivary and serum pellicles on C. albicans, Streptococcus mutans, S. sanguis, Lactobacillus and Actinomyces colonization on type I collagen, a major organic component of periodontal ligaments. The colonization potential of two isolates each of C. albicans, S. mutans and S. sanguis, and a single isolate each of Lactobacillus and Actinomyces to uncoated (control), saliva-coated or serum-coated type I collagen plates (surface area 143 mm2, Cell Disk; Sumitomo, Tokyo, Japan) was examined using a bioluminescent adenosine triphosphate assay based on firefly luciferase-luciferin system. The results revealed that with mutans streptococci, a saliva pellicle was significantly more effective in promoting bacterial colonization compared with the pellicle-free collagen disc, and the serum-coated sample significantly inhibited the colonization of streptococci (ANOVA; P < 0·01). In contrast, in the case of C. albicans, Lactobacillus and Actinomyces isolates, a serum pellicle was significantly more effective in promoting the colonization, followed by saliva pellicle and uncoated specimen (ANOVA; P < 0·01). These results suggested that crevicular fluid rich in seruminous components would promote the colonization of Candida, Lactobacillus and Actinomyces on type I collagen as opposed to streptococci which showed greater avidity to saliva-coated collagen.

Original languageEnglish
Pages (from-to)767-774
Number of pages8
JournalJournal of Oral Rehabilitation
Volume33
Issue number10
DOIs
Publication statusPublished - Oct 1 2006

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Actinomyces
Lactobacillus
Collagen Type I
Candida albicans
Saliva
Streptococcus mutans
Streptococcus
Serum
Firefly Luciferin
Analysis of Variance
Dental Pellicle
Collagen
Firefly Luciferases
Aspiration Pneumonia
Periodontal Ligament
Tokyo
Infection
Candida
Japan
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Nikawa, H., Egusa, H., Yamashiro, H., Nishimura, M., Makihira, S., Jin, C., ... Hamada, T. (2006). The effect of saliva or serum on bacterial and Candida albicans colonization on type I collagen. Journal of Oral Rehabilitation, 33(10), 767-774. https://doi.org/10.1046/j.0305-182X.2003.01149.x

The effect of saliva or serum on bacterial and Candida albicans colonization on type I collagen. / Nikawa, H.; Egusa, H.; Yamashiro, H.; Nishimura, M.; Makihira, S.; Jin, C.; Fukushima, H.; Hamada, T.

In: Journal of Oral Rehabilitation, Vol. 33, No. 10, 01.10.2006, p. 767-774.

Research output: Contribution to journalArticle

Nikawa, H, Egusa, H, Yamashiro, H, Nishimura, M, Makihira, S, Jin, C, Fukushima, H & Hamada, T 2006, 'The effect of saliva or serum on bacterial and Candida albicans colonization on type I collagen', Journal of Oral Rehabilitation, vol. 33, no. 10, pp. 767-774. https://doi.org/10.1046/j.0305-182X.2003.01149.x
Nikawa, H. ; Egusa, H. ; Yamashiro, H. ; Nishimura, M. ; Makihira, S. ; Jin, C. ; Fukushima, H. ; Hamada, T. / The effect of saliva or serum on bacterial and Candida albicans colonization on type I collagen. In: Journal of Oral Rehabilitation. 2006 ; Vol. 33, No. 10. pp. 767-774.
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