The effect of temperature on the viability of deformed cells

Hiroshi Takamatsu, Norihiko Kumagae, Boris Rubinsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Recent studies show that during slow freezing of cells, inaddition to chemical damage, the cells may be also injured bymechanical damage induced by ice crystal compression. A newexperimental procedure is employed to develop a quantitativeunderstanding of cell destruction by deformation with twoparallel surfaces. The viability of deformed cells (prostaticcarcinoma cells, 17.5 pm in mean diameter) is measured at 0°C, 23 "C and 37 °C, and six different nominal gap sizesbetween 32 pm and 3.5 pm. The relation between measuredviability and gap size at 0 °C and 23 °C are identical but theviability decreases at 37 °C. This may suggest that deformationdamage is not related to the mechanical properties of the lipidmembrane but the deformation of the cytoskeleton.

Original languageEnglish
Title of host publicationAdvances in Heat and Mass Transfer in Biotechnology
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages55-58
Number of pages4
ISBN (Electronic)9780791819296
DOIs
Publication statusPublished - 2000
EventASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000 - Orlando, United States
Duration: Nov 5 2000Nov 10 2000

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume2000-F

Conference

ConferenceASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000
Country/TerritoryUnited States
CityOrlando
Period11/5/0011/10/00

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

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