The effects of unsaturated fatty acids on lipid metabolism in HepG2 cells

Motoyuki Kohjima, Munechika Enjoji, Nobito Higuchi, Masaki Kato, Kazuhiro Kotoh, Manabu Nakashima, Makoto Nakamuta

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We investigated the effects of stearic acid (saturated), oleic acid (monounsaturated), linoleic acid (n-6 polyunsaturated), and α-linolenic acid (n-3 polyunsaturated) on lipid metabolism in a hepatocyte-derived cell line, HepG2. HepG2 cells were cultured in medium supplemented with either stearic acid (0.1% w/v), oleic acid (0.1% v/v), linoleic acid (0.1% v/v), or α-linolenic acid (0.1% v/v). After 24 h, expression of lipid metabolism-associated genes was evaluated by real-time PCR. α-Linolenic acid showed a suppressive effect on the hepatic fatty acid de novo synthesis and fatty acid oxidation pathways, while linoleic acid also showed a tendency to suppress these pathways although the effect was weaker. Moreover, α-linolenic acid enhanced the expression of enzymes associated with reactive oxygen species (ROS) elimination. In contrast, oleic acid tended to promote fatty acid synthesis and oxidation. In conclusion, α-linolenic acid and linoleic acid may be expected to ameliorate hepatic steatosis by downregulating fatty acid de novo synthesis and fatty acid oxidation, and by upregulating ROS elimination enzymes. Oleic acid had no distinct effects for improving steatosis or oxidative stress.

Original languageEnglish
Pages (from-to)6-9
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume45
Issue number1-2
DOIs
Publication statusPublished - Feb 1 2009

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Hep G2 Cells
Unsaturated Fatty Acids
Lipid Metabolism
alpha-Linolenic Acid
Oleic Acid
Fatty Acids
Linoleic Acid
Reactive Oxygen Species
Liver
Enzymes
Real-Time Polymerase Chain Reaction
Hepatocytes
Oxidative Stress
Down-Regulation
Cell Line
Genes
stearic acid

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

The effects of unsaturated fatty acids on lipid metabolism in HepG2 cells. / Kohjima, Motoyuki; Enjoji, Munechika; Higuchi, Nobito; Kato, Masaki; Kotoh, Kazuhiro; Nakashima, Manabu; Nakamuta, Makoto.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 45, No. 1-2, 01.02.2009, p. 6-9.

Research output: Contribution to journalArticle

Kohjima, Motoyuki ; Enjoji, Munechika ; Higuchi, Nobito ; Kato, Masaki ; Kotoh, Kazuhiro ; Nakashima, Manabu ; Nakamuta, Makoto. / The effects of unsaturated fatty acids on lipid metabolism in HepG2 cells. In: In Vitro Cellular and Developmental Biology - Animal. 2009 ; Vol. 45, No. 1-2. pp. 6-9.
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