The endosome-lysosome system in the absorptive cells of goldfish hindgut

Hiroshi Iida, Yosaburo Shibata, Torao Yamamoto

Research output: Contribution to journalArticle

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Abstract

The vacuolar system in the absorptive cells of the goldfish hindgut was studied by rapid freeze-substituted and cytochemical techniques. The apical cytoplasm of the absorptive cells contained two types of vacuoles: endosomes and lysosomes. The former were characterized by an absence of acid phosphatase activity, a dot-like distribution of material at the peripheral rim, the labelling of the inner surface with horseradish peroxidase (HRP), and by frequent connections to cytoplasmic tubules (CT), which were also free of acid phosphatase activity. The latter vacuole was preferentially located in the deeper cytoplasm and was characterized by the presence of acid phosphatase activity, an electron-dense interior matrix, a peripheral electron-lucent region (a halo), and by the detachment of HRP from the inner surface. Connections between CTs and these latter vacuoles were rarely seen. In the deeper cytoplasm, fusion between endosomes and lysosomes was sometimes observed. These results suggest that the vacuoles which are associated with CTs are endosomes, but not lysosomes, and that internalized materials are transported through the endosome-lysosome system to a giant food vacuole in the cell.

Original languageEnglish
Pages (from-to)449-452
Number of pages4
JournalCell and tissue research
Volume243
Issue number2
DOIs
Publication statusPublished - Feb 1 1986

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Goldfish
Endosomes
Vacuoles
Lysosomes
Acid Phosphatase
Cytoplasm
Horseradish Peroxidase
Electrons
Food

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

The endosome-lysosome system in the absorptive cells of goldfish hindgut. / Iida, Hiroshi; Shibata, Yosaburo; Yamamoto, Torao.

In: Cell and tissue research, Vol. 243, No. 2, 01.02.1986, p. 449-452.

Research output: Contribution to journalArticle

Iida, Hiroshi ; Shibata, Yosaburo ; Yamamoto, Torao. / The endosome-lysosome system in the absorptive cells of goldfish hindgut. In: Cell and tissue research. 1986 ; Vol. 243, No. 2. pp. 449-452.
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