Abstract
Myristate (C14:0) is an essential multi-functional fatty acid in a variety of organisms. We found that C14:0, but not other fatty acids, causes severe growth retardation in Hpelo1Δ and Hpelo2Δ mutants of the yeast Hansenula polymorpha, defective in elongation of very long-chain fatty acids. While transcription of HpELO1 and HpELO2 is transiently increased by C14:0, this was not found to be responsible for the growth retardation. Transcription of HpFAS1 and HpFAS2 encoding fatty acid synthase is repressed by C14:0, but this repression was also not found to be responsible for growth retardation. A screen for suppressors that resulted in restored growth of the Hpelo1Δ disruptant on media containing C14:0 identified two types of suppressors. One exhibited a defect in C14:0 uptake while the other did not. Molecular genetic and genomic analysis of these suppressor mutations is anticipated to shed new light on the processes of fatty acid transport and the crucial role of C14:0 in the growth of eukaryotic organisms.
| Original language | English |
|---|---|
| Pages (from-to) | 297-304 |
| Number of pages | 8 |
| Journal | Archives of Microbiology |
| Volume | 189 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2008 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Microbiology
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The essential fatty acid myristate causes severe growth retardation in Hpelo disruptants of the yeast Hansenula polymorpha. / Prasitchoke, Phatthanon; Kaneko, Yoshinobu; Bamba, Takeshi; Fukusaki, Ei Ichiro; Kobayashi, Akio; Harashima, Satoshi.
In: Archives of Microbiology, Vol. 189, No. 4, 01.04.2008, p. 297-304.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The essential fatty acid myristate causes severe growth retardation in Hpelo disruptants of the yeast Hansenula polymorpha
AU - Prasitchoke, Phatthanon
AU - Kaneko, Yoshinobu
AU - Bamba, Takeshi
AU - Fukusaki, Ei Ichiro
AU - Kobayashi, Akio
AU - Harashima, Satoshi
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Myristate (C14:0) is an essential multi-functional fatty acid in a variety of organisms. We found that C14:0, but not other fatty acids, causes severe growth retardation in Hpelo1Δ and Hpelo2Δ mutants of the yeast Hansenula polymorpha, defective in elongation of very long-chain fatty acids. While transcription of HpELO1 and HpELO2 is transiently increased by C14:0, this was not found to be responsible for the growth retardation. Transcription of HpFAS1 and HpFAS2 encoding fatty acid synthase is repressed by C14:0, but this repression was also not found to be responsible for growth retardation. A screen for suppressors that resulted in restored growth of the Hpelo1Δ disruptant on media containing C14:0 identified two types of suppressors. One exhibited a defect in C14:0 uptake while the other did not. Molecular genetic and genomic analysis of these suppressor mutations is anticipated to shed new light on the processes of fatty acid transport and the crucial role of C14:0 in the growth of eukaryotic organisms.
AB - Myristate (C14:0) is an essential multi-functional fatty acid in a variety of organisms. We found that C14:0, but not other fatty acids, causes severe growth retardation in Hpelo1Δ and Hpelo2Δ mutants of the yeast Hansenula polymorpha, defective in elongation of very long-chain fatty acids. While transcription of HpELO1 and HpELO2 is transiently increased by C14:0, this was not found to be responsible for the growth retardation. Transcription of HpFAS1 and HpFAS2 encoding fatty acid synthase is repressed by C14:0, but this repression was also not found to be responsible for growth retardation. A screen for suppressors that resulted in restored growth of the Hpelo1Δ disruptant on media containing C14:0 identified two types of suppressors. One exhibited a defect in C14:0 uptake while the other did not. Molecular genetic and genomic analysis of these suppressor mutations is anticipated to shed new light on the processes of fatty acid transport and the crucial role of C14:0 in the growth of eukaryotic organisms.
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UR - http://www.scopus.com/inward/citedby.url?scp=43349086590&partnerID=8YFLogxK
U2 - 10.1007/s00203-007-0317-7
DO - 10.1007/s00203-007-0317-7
M3 - Article
C2 - 17992514
AN - SCOPUS:43349086590
VL - 189
SP - 297
EP - 304
JO - Archives of Microbiology
JF - Archives of Microbiology
SN - 0302-8933
IS - 4
ER -