The ethylene signal mediates induction of GmATG8i in soybean plants under starvationstress

Munehiro Okuda, Myint Phyu Sin Htwe Nang, Kaori Oshima, Yushi Ishibashi, Shao Hui Zheng, Takashi Yuasa, Mari Iwaya-Inoue

Research output: Contribution to journalArticle

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Abstract

In higher plants, autophagy-related genes (ATGs) appear to play important roles in development, senescence, and starvation responses. Hormone signals underlying starvation-induced gene expression are involved in the expression of ATGs. An effect of starvation stress on the expression of ATGs and ethylene-related genes in young seedlings of soybean (Glycine max [L.] Merr. cv. Fukuyutaka) was analyzed. Reverse transcription-polymerase chain reaction (RT-PCR) showed that the expression levels of GmATG8i and GmATG4 increase in a starvation medium, but at a null or marginal level in a sucrose/nitrate-rich medium. The expression of GmACC synthase and GmERF are also upregulated in the starvation medium. In addition, immunoblot revealed that ethylene insensitive 3 (Ein3), an ethylene-induced transcription factor are accumulated in seedlings subjected to severe starvation stress. These results indicate that starvation stress stimulates the expression of GmATG8i and ethylene signal-related genes. Since the ethylene signal is involved in senescence and various environmental stresses, it is possible that starvation stress-induced autophagy is partly mediated by the ethylene signaling.

Original languageEnglish
Pages (from-to)1408-1412
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number7
DOIs
Publication statusPublished - Aug 1 2011

Fingerprint

Starvation
Soybeans
Genes
Autophagy
Seedlings
Polymerase chain reaction
Transcription
Gene expression
Nitrates
Glycine
Sucrose
ethylene
Transcription Factors
Hormones
Reverse Transcription
Gene Expression
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

The ethylene signal mediates induction of GmATG8i in soybean plants under starvationstress. / Okuda, Munehiro; Nang, Myint Phyu Sin Htwe; Oshima, Kaori; Ishibashi, Yushi; Zheng, Shao Hui; Yuasa, Takashi; Iwaya-Inoue, Mari.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 7, 01.08.2011, p. 1408-1412.

Research output: Contribution to journalArticle

Okuda, Munehiro ; Nang, Myint Phyu Sin Htwe ; Oshima, Kaori ; Ishibashi, Yushi ; Zheng, Shao Hui ; Yuasa, Takashi ; Iwaya-Inoue, Mari. / The ethylene signal mediates induction of GmATG8i in soybean plants under starvationstress. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 7. pp. 1408-1412.
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