TY - JOUR
T1 - The ethylene signal mediates induction of GmATG8i in soybean plants under starvationstress
AU - Okuda, Munehiro
AU - Nang, Myint Phyu Sin Htwe
AU - Oshima, Kaori
AU - Ishibashi, Yushi
AU - Zheng, Shao Hui
AU - Yuasa, Takashi
AU - Iwaya-Inoue, Mari
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Culture, Science, and Technology of Japan (no. 19380022) to T.Y. We are grateful to Ms. K. Inoue and Ms. S. Son for careful reading of the manuscript.
PY - 2011
Y1 - 2011
N2 - In higher plants, autophagy-related genes (ATGs) appear to play important roles in development, senescence, and starvation responses. Hormone signals underlying starvation-induced gene expression are involved in the expression of ATGs. An effect of starvation stress on the expression of ATGs and ethylene-related genes in young seedlings of soybean (Glycine max [L.] Merr. cv. Fukuyutaka) was analyzed. Reverse transcription-polymerase chain reaction (RT-PCR) showed that the expression levels of GmATG8i and GmATG4 increase in a starvation medium, but at a null or marginal level in a sucrose/nitrate-rich medium. The expression of GmACC synthase and GmERF are also upregulated in the starvation medium. In addition, immunoblot revealed that ethylene insensitive 3 (Ein3), an ethylene-induced transcription factor are accumulated in seedlings subjected to severe starvation stress. These results indicate that starvation stress stimulates the expression of GmATG8i and ethylene signal-related genes. Since the ethylene signal is involved in senescence and various environmental stresses, it is possible that starvation stress-induced autophagy is partly mediated by the ethylene signaling.
AB - In higher plants, autophagy-related genes (ATGs) appear to play important roles in development, senescence, and starvation responses. Hormone signals underlying starvation-induced gene expression are involved in the expression of ATGs. An effect of starvation stress on the expression of ATGs and ethylene-related genes in young seedlings of soybean (Glycine max [L.] Merr. cv. Fukuyutaka) was analyzed. Reverse transcription-polymerase chain reaction (RT-PCR) showed that the expression levels of GmATG8i and GmATG4 increase in a starvation medium, but at a null or marginal level in a sucrose/nitrate-rich medium. The expression of GmACC synthase and GmERF are also upregulated in the starvation medium. In addition, immunoblot revealed that ethylene insensitive 3 (Ein3), an ethylene-induced transcription factor are accumulated in seedlings subjected to severe starvation stress. These results indicate that starvation stress stimulates the expression of GmATG8i and ethylene signal-related genes. Since the ethylene signal is involved in senescence and various environmental stresses, it is possible that starvation stress-induced autophagy is partly mediated by the ethylene signaling.
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U2 - 10.1271/bbb.110086
DO - 10.1271/bbb.110086
M3 - Article
C2 - 21737912
AN - SCOPUS:79960811470
SN - 0916-8451
VL - 75
SP - 1408
EP - 1412
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -