The expression and function of thymosin beta 10 in tooth germ development

Maho Shiotsuka, Hiroko Wada, Tamotsu Kiyoshima, Kengo Nagata, Hiroaki Fujiwara, Makiko Kihara, Kana Hasegawa, Hirotaka Someya, Ichiro Takahashi, Hidetaka Sakai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study presents the expression pattern and functions of thymosin beta 10 (Tβ10), a Tβ4 homologue during the development of mouse lower first molars. An in situ signal of Tβ10 was detected on embryonic day 10.5 (E10.5)-E15.5 mainly in dental mesenchymal cells as well as in dental epithelial cells, while Tβ4 was expressed in dental epithelial cells. In the late bell stage, preodontoblasts with strong Tβ10 expression and preameloblasts with strong Tβ4 expression exhibited face-to-face localization, suggesting that an intimate cell-cell interaction might exist between preodontoblasts and preameloblasts to form dentin and enamel matrices. A strong Tβ10 signal was found in odontoblasts in the lateral side of the dental pulp and in Hertwig's epithelial root sheath, thus suggesting that Tβ10 participates in the formation of the outline of the tooth root. An inhibition assay using Tβ10-siRNA in E11.0 mandibles showed significant growth inhibition in the tooth germ. The Tβ10-siRNA-treated E15.0 tooth germ also showed significant developmental arrest. The number of Ki67-positive cells significantly decreased in the Tβ10-siRNA-treated mandibles. The cellular proliferative activity was also significantly suppressed in Tβ10-siRNA-treated cultured mouse dental pulpal and epithelial cells. These results indicate that developmental arrest of the tooth germ might be caused by a reduction in cell proliferative activity. The stage-specific temporal and spatial expression pattern of Tβ10 in the developing tooth germ is indicative of multiple functions of Tβ10 in the developmental course from initiation to root formation of the tooth germ.

Original languageEnglish
Pages (from-to)895-905
Number of pages11
JournalInternational Journal of Developmental Biology
Volume57
Issue number11-12
DOIs
Publication statusPublished - Jan 1 2013

Fingerprint

Tooth Germ
Small Interfering RNA
Tooth
Epithelial Cells
Mandible
thymosin beta(10)
Odontoblasts
Tooth Root
Dental Pulp
Dentin
Dental Enamel
Cell Communication

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Cite this

The expression and function of thymosin beta 10 in tooth germ development. / Shiotsuka, Maho; Wada, Hiroko; Kiyoshima, Tamotsu; Nagata, Kengo; Fujiwara, Hiroaki; Kihara, Makiko; Hasegawa, Kana; Someya, Hirotaka; Takahashi, Ichiro; Sakai, Hidetaka.

In: International Journal of Developmental Biology, Vol. 57, No. 11-12, 01.01.2013, p. 895-905.

Research output: Contribution to journalArticle

Shiotsuka, Maho ; Wada, Hiroko ; Kiyoshima, Tamotsu ; Nagata, Kengo ; Fujiwara, Hiroaki ; Kihara, Makiko ; Hasegawa, Kana ; Someya, Hirotaka ; Takahashi, Ichiro ; Sakai, Hidetaka. / The expression and function of thymosin beta 10 in tooth germ development. In: International Journal of Developmental Biology. 2013 ; Vol. 57, No. 11-12. pp. 895-905.
@article{1d6b7f8b5981494d8c5b81009eccae05,
title = "The expression and function of thymosin beta 10 in tooth germ development",
abstract = "This study presents the expression pattern and functions of thymosin beta 10 (Tβ10), a Tβ4 homologue during the development of mouse lower first molars. An in situ signal of Tβ10 was detected on embryonic day 10.5 (E10.5)-E15.5 mainly in dental mesenchymal cells as well as in dental epithelial cells, while Tβ4 was expressed in dental epithelial cells. In the late bell stage, preodontoblasts with strong Tβ10 expression and preameloblasts with strong Tβ4 expression exhibited face-to-face localization, suggesting that an intimate cell-cell interaction might exist between preodontoblasts and preameloblasts to form dentin and enamel matrices. A strong Tβ10 signal was found in odontoblasts in the lateral side of the dental pulp and in Hertwig's epithelial root sheath, thus suggesting that Tβ10 participates in the formation of the outline of the tooth root. An inhibition assay using Tβ10-siRNA in E11.0 mandibles showed significant growth inhibition in the tooth germ. The Tβ10-siRNA-treated E15.0 tooth germ also showed significant developmental arrest. The number of Ki67-positive cells significantly decreased in the Tβ10-siRNA-treated mandibles. The cellular proliferative activity was also significantly suppressed in Tβ10-siRNA-treated cultured mouse dental pulpal and epithelial cells. These results indicate that developmental arrest of the tooth germ might be caused by a reduction in cell proliferative activity. The stage-specific temporal and spatial expression pattern of Tβ10 in the developing tooth germ is indicative of multiple functions of Tβ10 in the developmental course from initiation to root formation of the tooth germ.",
author = "Maho Shiotsuka and Hiroko Wada and Tamotsu Kiyoshima and Kengo Nagata and Hiroaki Fujiwara and Makiko Kihara and Kana Hasegawa and Hirotaka Someya and Ichiro Takahashi and Hidetaka Sakai",
year = "2013",
month = "1",
day = "1",
doi = "10.1387/ijdb.120240hs",
language = "English",
volume = "57",
pages = "895--905",
journal = "International Journal of Developmental Biology",
issn = "0214-6282",
publisher = "University of the Basque Country Press",
number = "11-12",

}

TY - JOUR

T1 - The expression and function of thymosin beta 10 in tooth germ development

AU - Shiotsuka, Maho

AU - Wada, Hiroko

AU - Kiyoshima, Tamotsu

AU - Nagata, Kengo

AU - Fujiwara, Hiroaki

AU - Kihara, Makiko

AU - Hasegawa, Kana

AU - Someya, Hirotaka

AU - Takahashi, Ichiro

AU - Sakai, Hidetaka

PY - 2013/1/1

Y1 - 2013/1/1

N2 - This study presents the expression pattern and functions of thymosin beta 10 (Tβ10), a Tβ4 homologue during the development of mouse lower first molars. An in situ signal of Tβ10 was detected on embryonic day 10.5 (E10.5)-E15.5 mainly in dental mesenchymal cells as well as in dental epithelial cells, while Tβ4 was expressed in dental epithelial cells. In the late bell stage, preodontoblasts with strong Tβ10 expression and preameloblasts with strong Tβ4 expression exhibited face-to-face localization, suggesting that an intimate cell-cell interaction might exist between preodontoblasts and preameloblasts to form dentin and enamel matrices. A strong Tβ10 signal was found in odontoblasts in the lateral side of the dental pulp and in Hertwig's epithelial root sheath, thus suggesting that Tβ10 participates in the formation of the outline of the tooth root. An inhibition assay using Tβ10-siRNA in E11.0 mandibles showed significant growth inhibition in the tooth germ. The Tβ10-siRNA-treated E15.0 tooth germ also showed significant developmental arrest. The number of Ki67-positive cells significantly decreased in the Tβ10-siRNA-treated mandibles. The cellular proliferative activity was also significantly suppressed in Tβ10-siRNA-treated cultured mouse dental pulpal and epithelial cells. These results indicate that developmental arrest of the tooth germ might be caused by a reduction in cell proliferative activity. The stage-specific temporal and spatial expression pattern of Tβ10 in the developing tooth germ is indicative of multiple functions of Tβ10 in the developmental course from initiation to root formation of the tooth germ.

AB - This study presents the expression pattern and functions of thymosin beta 10 (Tβ10), a Tβ4 homologue during the development of mouse lower first molars. An in situ signal of Tβ10 was detected on embryonic day 10.5 (E10.5)-E15.5 mainly in dental mesenchymal cells as well as in dental epithelial cells, while Tβ4 was expressed in dental epithelial cells. In the late bell stage, preodontoblasts with strong Tβ10 expression and preameloblasts with strong Tβ4 expression exhibited face-to-face localization, suggesting that an intimate cell-cell interaction might exist between preodontoblasts and preameloblasts to form dentin and enamel matrices. A strong Tβ10 signal was found in odontoblasts in the lateral side of the dental pulp and in Hertwig's epithelial root sheath, thus suggesting that Tβ10 participates in the formation of the outline of the tooth root. An inhibition assay using Tβ10-siRNA in E11.0 mandibles showed significant growth inhibition in the tooth germ. The Tβ10-siRNA-treated E15.0 tooth germ also showed significant developmental arrest. The number of Ki67-positive cells significantly decreased in the Tβ10-siRNA-treated mandibles. The cellular proliferative activity was also significantly suppressed in Tβ10-siRNA-treated cultured mouse dental pulpal and epithelial cells. These results indicate that developmental arrest of the tooth germ might be caused by a reduction in cell proliferative activity. The stage-specific temporal and spatial expression pattern of Tβ10 in the developing tooth germ is indicative of multiple functions of Tβ10 in the developmental course from initiation to root formation of the tooth germ.

UR - http://www.scopus.com/inward/record.url?scp=84890327364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890327364&partnerID=8YFLogxK

U2 - 10.1387/ijdb.120240hs

DO - 10.1387/ijdb.120240hs

M3 - Article

VL - 57

SP - 895

EP - 905

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

SN - 0214-6282

IS - 11-12

ER -