The expression and sequences of T cell antigen receptor β-chain genes in the thymus at an early stage after sublethal irradiation

H. Yuuki, Yasunobu Yoshikai, K. Kishihara, G. Matsuzaki, K. Ayukawa, K. Nomoto

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The sequential appearance of the thymocyte subpopulations and TCR gene messages occurred in the thymus of AKR mice (H-2(k), M1sa) from 7 to 14 days after sublethal irradiation. The thymocytes on day 7 after irradiation were composed of a large number of CD4+CD8+ blast-like cells and a relatively high proportion of CD4-CD8- cells (15 to 25%) but few CD3(high)CD4+CD8-/CD4-CD8+ cells. Approximately 22% of the CD4-CD8- cells were CD3(high) and -27% of the CD3(high)CD4-CD8- cells (-6% of whole CD4-CD8- cells) were F23.1+. The thymocytes on day 7 expressed a large amount of γ- and δ-chain gene transcripts but reduced levels of α- and β-chain gene transcripts. The V gene repertoire of 18 functional β-chain cDNA derived from the thymocytes on day 7 was compared with those of 20 functional β-chain cDNA derived from the thymocytes on day 14 which were composed of a large number of CD3(low)CD4+CD8+ small-sized cells and a small number of CD3(high)CD4+CD8- cells. It is noteworthy that the distribution of Vβ genes expressed in the thymocytes on day 7 was much the same as that in the thymocytes on day 14 but significantly different from that in normal BALB/c thymocytes as previously described. Interestingly, neither V(β8.1) nor V(β6) genes, which are important for recognition of the product of the M1sa locus, was detected in these two cDNA libraries. These results suggest that clonal selection of TCR V(β) repertoire, irrespective of positive or negative selection, appears to occur at the early stage of T cell differentiation, i.e., on the blast-like CD4+CD8+ thymocytes.

    Original languageEnglish
    Pages (from-to)3683-3691
    Number of pages9
    JournalJournal of Immunology
    Volume142
    Issue number10
    Publication statusPublished - Jan 1 1989

    Fingerprint

    Thymocytes
    T-Cell Antigen Receptor
    Thymus Gland
    Genes
    Complementary DNA
    Inbred AKR Mouse
    Gene Library
    Cell Differentiation
    T-Lymphocytes

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Yuuki, H., Yoshikai, Y., Kishihara, K., Matsuzaki, G., Ayukawa, K., & Nomoto, K. (1989). The expression and sequences of T cell antigen receptor β-chain genes in the thymus at an early stage after sublethal irradiation. Journal of Immunology, 142(10), 3683-3691.

    The expression and sequences of T cell antigen receptor β-chain genes in the thymus at an early stage after sublethal irradiation. / Yuuki, H.; Yoshikai, Yasunobu; Kishihara, K.; Matsuzaki, G.; Ayukawa, K.; Nomoto, K.

    In: Journal of Immunology, Vol. 142, No. 10, 01.01.1989, p. 3683-3691.

    Research output: Contribution to journalArticle

    Yuuki, H, Yoshikai, Y, Kishihara, K, Matsuzaki, G, Ayukawa, K & Nomoto, K 1989, 'The expression and sequences of T cell antigen receptor β-chain genes in the thymus at an early stage after sublethal irradiation', Journal of Immunology, vol. 142, no. 10, pp. 3683-3691.
    Yuuki H, Yoshikai Y, Kishihara K, Matsuzaki G, Ayukawa K, Nomoto K. The expression and sequences of T cell antigen receptor β-chain genes in the thymus at an early stage after sublethal irradiation. Journal of Immunology. 1989 Jan 1;142(10):3683-3691.
    Yuuki, H. ; Yoshikai, Yasunobu ; Kishihara, K. ; Matsuzaki, G. ; Ayukawa, K. ; Nomoto, K. / The expression and sequences of T cell antigen receptor β-chain genes in the thymus at an early stage after sublethal irradiation. In: Journal of Immunology. 1989 ; Vol. 142, No. 10. pp. 3683-3691.
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    abstract = "The sequential appearance of the thymocyte subpopulations and TCR gene messages occurred in the thymus of AKR mice (H-2(k), M1sa) from 7 to 14 days after sublethal irradiation. The thymocytes on day 7 after irradiation were composed of a large number of CD4+CD8+ blast-like cells and a relatively high proportion of CD4-CD8- cells (15 to 25{\%}) but few CD3(high)CD4+CD8-/CD4-CD8+ cells. Approximately 22{\%} of the CD4-CD8- cells were CD3(high) and -27{\%} of the CD3(high)CD4-CD8- cells (-6{\%} of whole CD4-CD8- cells) were F23.1+. The thymocytes on day 7 expressed a large amount of γ- and δ-chain gene transcripts but reduced levels of α- and β-chain gene transcripts. The V gene repertoire of 18 functional β-chain cDNA derived from the thymocytes on day 7 was compared with those of 20 functional β-chain cDNA derived from the thymocytes on day 14 which were composed of a large number of CD3(low)CD4+CD8+ small-sized cells and a small number of CD3(high)CD4+CD8- cells. It is noteworthy that the distribution of Vβ genes expressed in the thymocytes on day 7 was much the same as that in the thymocytes on day 14 but significantly different from that in normal BALB/c thymocytes as previously described. Interestingly, neither V(β8.1) nor V(β6) genes, which are important for recognition of the product of the M1sa locus, was detected in these two cDNA libraries. These results suggest that clonal selection of TCR V(β) repertoire, irrespective of positive or negative selection, appears to occur at the early stage of T cell differentiation, i.e., on the blast-like CD4+CD8+ thymocytes.",
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