TY - JOUR
T1 - The expression of SF-1/Ad4BP is related to the process of luteinization in the marmoset (Callithrix jacchus) ovary
AU - Wehrenberg, Uwe
AU - Wulff, Christine
AU - Husen, Bettina
AU - Morohashi, Ken Ichirou
AU - Rune, Gabriele M.
PY - 1997
Y1 - 1997
N2 - Expression of the nuclear receptor, steroidogenic factor-1 (SF-1/Ad4BP), was studied in a primate (marmoset) ovary using immunohistochemical, RT-PCR, and immunoblot techniques. The periovulatory phase was compared with the luteal phase. With PCR we found a marmoset homolog of SF-1/Ad4BP to be expressed in ovarian and other steroidogenic tissues. Characteristically, the periovulatory ovaries consisted of growing (nonluteinized) small follicles together with large luteinizing follicles and many corpora lutea accessoria (Clas), which had developed from atretic large follicles. During the luteal phase, true corpora lutea (Cls) were additionally found. In general, we found that small follicles were devoid of any immunoreactivity of SF-1/Ad4BP. In large follicles, the luteinizing theca and granulosa cells express SF-1/Ad4BP. All luteal cells of Clas showed a nuclear staining in both ovary types. In Cls, only a few luteal cells were positive. Large follicles of different sizes showed no differences in expression level, as evidenced by immunoblot analysis. Our results indicate that SF-1/Ad4BP participates in the activation of gene transcription during the onset of luteinization and that Clas are essential for ovarian luteal function.
AB - Expression of the nuclear receptor, steroidogenic factor-1 (SF-1/Ad4BP), was studied in a primate (marmoset) ovary using immunohistochemical, RT-PCR, and immunoblot techniques. The periovulatory phase was compared with the luteal phase. With PCR we found a marmoset homolog of SF-1/Ad4BP to be expressed in ovarian and other steroidogenic tissues. Characteristically, the periovulatory ovaries consisted of growing (nonluteinized) small follicles together with large luteinizing follicles and many corpora lutea accessoria (Clas), which had developed from atretic large follicles. During the luteal phase, true corpora lutea (Cls) were additionally found. In general, we found that small follicles were devoid of any immunoreactivity of SF-1/Ad4BP. In large follicles, the luteinizing theca and granulosa cells express SF-1/Ad4BP. All luteal cells of Clas showed a nuclear staining in both ovary types. In Cls, only a few luteal cells were positive. Large follicles of different sizes showed no differences in expression level, as evidenced by immunoblot analysis. Our results indicate that SF-1/Ad4BP participates in the activation of gene transcription during the onset of luteinization and that Clas are essential for ovarian luteal function.
UR - http://www.scopus.com/inward/record.url?scp=0030970454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030970454&partnerID=8YFLogxK
U2 - 10.1007/s004180050120
DO - 10.1007/s004180050120
M3 - Article
C2 - 9151117
AN - SCOPUS:0030970454
VL - 107
SP - 345
EP - 350
JO - Zeitschrift für Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie
JF - Zeitschrift für Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie
SN - 0948-6143
IS - 4
ER -