The feasibility of using biopsy samples from esophageal cancer for comprehensive gene expression profiling

Masaaki Motoori, Ichiro Takemasa, Makoto Yamasaki, Takamichi Komori, Atsushi Takeno, Hiroshi Miyata, Shuji Takiguchi, Yoshiyuki Fujiwara, Takushi Yasuda, Masahiko Yano, Nariaki Matsuura, Kenichi Matsubara, Morito Monden, Masaki Mori

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Advanced esophageal cancer has been recently treated by multimodal therapy including preoperative chemotherapy or chemoradiotherapy and surgery. A biopsy sample provides a valuable specimen for understanding the biological characteristics of individual esophageal cancer. Pretreatment prediction of the response to chemotherapy or radiotherapy based on biological characteristics using biopsy samples is a desirable goal. In using biopsy samples for molecular analysis, there are two problems; the proportion of cancer cells and the intratumor heterogeneity. This study was conducted to investigate the feasibility of using endoscopic biopsy samples of esophageal squamous cell cancer (ESCC) for comprehensive gene expression profiling (GEP). Comprehensive GEP was performed in 40 bulky ESCC specimens and 10 normal esophageal epithelial specimens from patients who underwent esophageal resection and 52 endoscopic ESCC biopsy samples from 26 patients (two samples per one patient). Unsupervised hierarchical cluster analysis showed distinct profiles between the bulky ESCC specimens and normal epithelial specimens. Also, unsupervised hierarchical cluster analysis revealed distinct profiles between the biopsy ESCC samples and normal epithelial specimens. Moreover, a couple of biopsy samples taken from different locations of the same tumor were closely clustered together. That is, biopsy ESCC samples were distinguished from normal esophageal epithelial specimens and the intratumor heterogeneity of GEP was smaller than intertumor heterogeneity. GEP using biopsy ESCC samples is feasible and has the potential to represent the biological properties.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalInternational journal of oncology
Volume35
Issue number2
DOIs
Publication statusPublished - Sep 21 2009

Fingerprint

Neoplasm Genes
Gene Expression Profiling
Esophageal Neoplasms
Squamous Cell Neoplasms
Biopsy
Cluster Analysis
Drug Therapy
Chemoradiotherapy
Neoplasms
Radiotherapy

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

The feasibility of using biopsy samples from esophageal cancer for comprehensive gene expression profiling. / Motoori, Masaaki; Takemasa, Ichiro; Yamasaki, Makoto; Komori, Takamichi; Takeno, Atsushi; Miyata, Hiroshi; Takiguchi, Shuji; Fujiwara, Yoshiyuki; Yasuda, Takushi; Yano, Masahiko; Matsuura, Nariaki; Matsubara, Kenichi; Monden, Morito; Mori, Masaki.

In: International journal of oncology, Vol. 35, No. 2, 21.09.2009, p. 265-271.

Research output: Contribution to journalArticle

Motoori, M, Takemasa, I, Yamasaki, M, Komori, T, Takeno, A, Miyata, H, Takiguchi, S, Fujiwara, Y, Yasuda, T, Yano, M, Matsuura, N, Matsubara, K, Monden, M & Mori, M 2009, 'The feasibility of using biopsy samples from esophageal cancer for comprehensive gene expression profiling', International journal of oncology, vol. 35, no. 2, pp. 265-271. https://doi.org/10.3892/ijo_00000336
Motoori, Masaaki ; Takemasa, Ichiro ; Yamasaki, Makoto ; Komori, Takamichi ; Takeno, Atsushi ; Miyata, Hiroshi ; Takiguchi, Shuji ; Fujiwara, Yoshiyuki ; Yasuda, Takushi ; Yano, Masahiko ; Matsuura, Nariaki ; Matsubara, Kenichi ; Monden, Morito ; Mori, Masaki. / The feasibility of using biopsy samples from esophageal cancer for comprehensive gene expression profiling. In: International journal of oncology. 2009 ; Vol. 35, No. 2. pp. 265-271.
@article{aec96951d01e4d52a8f734d052a6bfb3,
title = "The feasibility of using biopsy samples from esophageal cancer for comprehensive gene expression profiling",
abstract = "Advanced esophageal cancer has been recently treated by multimodal therapy including preoperative chemotherapy or chemoradiotherapy and surgery. A biopsy sample provides a valuable specimen for understanding the biological characteristics of individual esophageal cancer. Pretreatment prediction of the response to chemotherapy or radiotherapy based on biological characteristics using biopsy samples is a desirable goal. In using biopsy samples for molecular analysis, there are two problems; the proportion of cancer cells and the intratumor heterogeneity. This study was conducted to investigate the feasibility of using endoscopic biopsy samples of esophageal squamous cell cancer (ESCC) for comprehensive gene expression profiling (GEP). Comprehensive GEP was performed in 40 bulky ESCC specimens and 10 normal esophageal epithelial specimens from patients who underwent esophageal resection and 52 endoscopic ESCC biopsy samples from 26 patients (two samples per one patient). Unsupervised hierarchical cluster analysis showed distinct profiles between the bulky ESCC specimens and normal epithelial specimens. Also, unsupervised hierarchical cluster analysis revealed distinct profiles between the biopsy ESCC samples and normal epithelial specimens. Moreover, a couple of biopsy samples taken from different locations of the same tumor were closely clustered together. That is, biopsy ESCC samples were distinguished from normal esophageal epithelial specimens and the intratumor heterogeneity of GEP was smaller than intertumor heterogeneity. GEP using biopsy ESCC samples is feasible and has the potential to represent the biological properties.",
author = "Masaaki Motoori and Ichiro Takemasa and Makoto Yamasaki and Takamichi Komori and Atsushi Takeno and Hiroshi Miyata and Shuji Takiguchi and Yoshiyuki Fujiwara and Takushi Yasuda and Masahiko Yano and Nariaki Matsuura and Kenichi Matsubara and Morito Monden and Masaki Mori",
year = "2009",
month = "9",
day = "21",
doi = "10.3892/ijo_00000336",
language = "English",
volume = "35",
pages = "265--271",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "2",

}

TY - JOUR

T1 - The feasibility of using biopsy samples from esophageal cancer for comprehensive gene expression profiling

AU - Motoori, Masaaki

AU - Takemasa, Ichiro

AU - Yamasaki, Makoto

AU - Komori, Takamichi

AU - Takeno, Atsushi

AU - Miyata, Hiroshi

AU - Takiguchi, Shuji

AU - Fujiwara, Yoshiyuki

AU - Yasuda, Takushi

AU - Yano, Masahiko

AU - Matsuura, Nariaki

AU - Matsubara, Kenichi

AU - Monden, Morito

AU - Mori, Masaki

PY - 2009/9/21

Y1 - 2009/9/21

N2 - Advanced esophageal cancer has been recently treated by multimodal therapy including preoperative chemotherapy or chemoradiotherapy and surgery. A biopsy sample provides a valuable specimen for understanding the biological characteristics of individual esophageal cancer. Pretreatment prediction of the response to chemotherapy or radiotherapy based on biological characteristics using biopsy samples is a desirable goal. In using biopsy samples for molecular analysis, there are two problems; the proportion of cancer cells and the intratumor heterogeneity. This study was conducted to investigate the feasibility of using endoscopic biopsy samples of esophageal squamous cell cancer (ESCC) for comprehensive gene expression profiling (GEP). Comprehensive GEP was performed in 40 bulky ESCC specimens and 10 normal esophageal epithelial specimens from patients who underwent esophageal resection and 52 endoscopic ESCC biopsy samples from 26 patients (two samples per one patient). Unsupervised hierarchical cluster analysis showed distinct profiles between the bulky ESCC specimens and normal epithelial specimens. Also, unsupervised hierarchical cluster analysis revealed distinct profiles between the biopsy ESCC samples and normal epithelial specimens. Moreover, a couple of biopsy samples taken from different locations of the same tumor were closely clustered together. That is, biopsy ESCC samples were distinguished from normal esophageal epithelial specimens and the intratumor heterogeneity of GEP was smaller than intertumor heterogeneity. GEP using biopsy ESCC samples is feasible and has the potential to represent the biological properties.

AB - Advanced esophageal cancer has been recently treated by multimodal therapy including preoperative chemotherapy or chemoradiotherapy and surgery. A biopsy sample provides a valuable specimen for understanding the biological characteristics of individual esophageal cancer. Pretreatment prediction of the response to chemotherapy or radiotherapy based on biological characteristics using biopsy samples is a desirable goal. In using biopsy samples for molecular analysis, there are two problems; the proportion of cancer cells and the intratumor heterogeneity. This study was conducted to investigate the feasibility of using endoscopic biopsy samples of esophageal squamous cell cancer (ESCC) for comprehensive gene expression profiling (GEP). Comprehensive GEP was performed in 40 bulky ESCC specimens and 10 normal esophageal epithelial specimens from patients who underwent esophageal resection and 52 endoscopic ESCC biopsy samples from 26 patients (two samples per one patient). Unsupervised hierarchical cluster analysis showed distinct profiles between the bulky ESCC specimens and normal epithelial specimens. Also, unsupervised hierarchical cluster analysis revealed distinct profiles between the biopsy ESCC samples and normal epithelial specimens. Moreover, a couple of biopsy samples taken from different locations of the same tumor were closely clustered together. That is, biopsy ESCC samples were distinguished from normal esophageal epithelial specimens and the intratumor heterogeneity of GEP was smaller than intertumor heterogeneity. GEP using biopsy ESCC samples is feasible and has the potential to represent the biological properties.

UR - http://www.scopus.com/inward/record.url?scp=69449085262&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69449085262&partnerID=8YFLogxK

U2 - 10.3892/ijo_00000336

DO - 10.3892/ijo_00000336

M3 - Article

C2 - 19578739

AN - SCOPUS:69449085262

VL - 35

SP - 265

EP - 271

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 2

ER -