The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes

T. Fujii, N. Tanaka, T. Yokoyama, O. Ninaki, T. Oshiki, A. Ohnuma, Y. Tazima, Yutaka Banno, M. Ajimura, K. Mita, M. Seki, F. Ohbayashi, T. Shimada, H. Abe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Bombyx mori is a female-heterogametic organism (female, ZW; male, ZZ) that appears to have a putative feminizing gene (Fem) on the W chromosome. The paternally transmitted mutant W chromosome, Df(p Sa + p W + od )Fem, derived from the translocation-carrying W chromosome (p Sa + p W + od ), is inert as femaleness determinant. Moreover, this Df(p Sa + p W + od )Fem chromosome has been thought to have a female-killing factor because no female larvae having the Df(p Sa + p W + od )Fem chromosome are produced. Initially, to investigate whether the Df(p Sa + p W + od )Fem chromosome contains any region of the W chromosome or not, we analyzed the presence or absence of 12 W-specific RAPD markers. The Df(p Sa + p W + od )Fem chromosome contained 3 of 12 W-specific RAPD markers. These results strongly indicate that the Df(p Sa + p W + od )Fem chromosome contains the region of the W chromosome. Moreover, by using phenotypic and molecular markers, we confirmed that the Df(p Sa + p W + od )Fem chromosome is connected with a partially deleted Z chromosome and that this fused chromosome behaves as a Z chromosome during male meiosis. Furthermore, we demonstrated that the ZZW-type triploid female having the Df(p Sa + p W + od )Fem chromosome is viable. Therefore, we concluded that the Df(p Sa + p W + od )Fem chromosome does not have a female-killing factor but that partial deletion of the Z chromosome causes the death of the ZW-type diploid female having the Df(p Sa + p W + od )Fem chromosome. Additionally, our results of detailed genetic analyses strongly indicate that the female-killing chromosome composed of the Df(p Sa + p W + od )Fem chromosome and deleted Z chromosome was generated by translocation between the Z chromosome and the translocation-carrying W chromosome, p Sa + p W + od.

Original languageEnglish
Pages (from-to)253-265
Number of pages13
JournalGenetica
Volume127
Issue number1-3
DOIs
Publication statusPublished - May 1 2006

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W chromosome
Z chromosome
Bombyx
Bombyx mori
silkworms
Chromosomes
chromosomes
triploidy
meiosis

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

Cite this

Fujii, T., Tanaka, N., Yokoyama, T., Ninaki, O., Oshiki, T., Ohnuma, A., ... Abe, H. (2006). The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes. Genetica, 127(1-3), 253-265. https://doi.org/10.1007/s10709-005-4147-8

The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes. / Fujii, T.; Tanaka, N.; Yokoyama, T.; Ninaki, O.; Oshiki, T.; Ohnuma, A.; Tazima, Y.; Banno, Yutaka; Ajimura, M.; Mita, K.; Seki, M.; Ohbayashi, F.; Shimada, T.; Abe, H.

In: Genetica, Vol. 127, No. 1-3, 01.05.2006, p. 253-265.

Research output: Contribution to journalArticle

Fujii, T, Tanaka, N, Yokoyama, T, Ninaki, O, Oshiki, T, Ohnuma, A, Tazima, Y, Banno, Y, Ajimura, M, Mita, K, Seki, M, Ohbayashi, F, Shimada, T & Abe, H 2006, 'The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes', Genetica, vol. 127, no. 1-3, pp. 253-265. https://doi.org/10.1007/s10709-005-4147-8
Fujii, T. ; Tanaka, N. ; Yokoyama, T. ; Ninaki, O. ; Oshiki, T. ; Ohnuma, A. ; Tazima, Y. ; Banno, Yutaka ; Ajimura, M. ; Mita, K. ; Seki, M. ; Ohbayashi, F. ; Shimada, T. ; Abe, H. / The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes. In: Genetica. 2006 ; Vol. 127, No. 1-3. pp. 253-265.
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abstract = "Bombyx mori is a female-heterogametic organism (female, ZW; male, ZZ) that appears to have a putative feminizing gene (Fem) on the W chromosome. The paternally transmitted mutant W chromosome, Df(p Sa + p W + od )Fem, derived from the translocation-carrying W chromosome (p Sa + p W + od ), is inert as femaleness determinant. Moreover, this Df(p Sa + p W + od )Fem chromosome has been thought to have a female-killing factor because no female larvae having the Df(p Sa + p W + od )Fem chromosome are produced. Initially, to investigate whether the Df(p Sa + p W + od )Fem chromosome contains any region of the W chromosome or not, we analyzed the presence or absence of 12 W-specific RAPD markers. The Df(p Sa + p W + od )Fem chromosome contained 3 of 12 W-specific RAPD markers. These results strongly indicate that the Df(p Sa + p W + od )Fem chromosome contains the region of the W chromosome. Moreover, by using phenotypic and molecular markers, we confirmed that the Df(p Sa + p W + od )Fem chromosome is connected with a partially deleted Z chromosome and that this fused chromosome behaves as a Z chromosome during male meiosis. Furthermore, we demonstrated that the ZZW-type triploid female having the Df(p Sa + p W + od )Fem chromosome is viable. Therefore, we concluded that the Df(p Sa + p W + od )Fem chromosome does not have a female-killing factor but that partial deletion of the Z chromosome causes the death of the ZW-type diploid female having the Df(p Sa + p W + od )Fem chromosome. Additionally, our results of detailed genetic analyses strongly indicate that the female-killing chromosome composed of the Df(p Sa + p W + od )Fem chromosome and deleted Z chromosome was generated by translocation between the Z chromosome and the translocation-carrying W chromosome, p Sa + p W + od.",
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AU - Tanaka, N.

AU - Yokoyama, T.

AU - Ninaki, O.

AU - Oshiki, T.

AU - Ohnuma, A.

AU - Tazima, Y.

AU - Banno, Yutaka

AU - Ajimura, M.

AU - Mita, K.

AU - Seki, M.

AU - Ohbayashi, F.

AU - Shimada, T.

AU - Abe, H.

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