The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes

T. Fujii, N. Tanaka, T. Yokoyama, O. Ninaki, T. Oshiki, A. Ohnuma, Y. Tazima, Y. Banno, M. Ajimura, K. Mita, M. Seki, F. Ohbayashi, T. Shimada, H. Abe

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13 Citations (Scopus)

Abstract

Bombyx mori is a female-heterogametic organism (female, ZW; male, ZZ) that appears to have a putative feminizing gene (Fem) on the W chromosome. The paternally transmitted mutant W chromosome, Df(p Sa + p W + od )Fem, derived from the translocation-carrying W chromosome (p Sa + p W + od ), is inert as femaleness determinant. Moreover, this Df(p Sa + p W + od )Fem chromosome has been thought to have a female-killing factor because no female larvae having the Df(p Sa + p W + od )Fem chromosome are produced. Initially, to investigate whether the Df(p Sa + p W + od )Fem chromosome contains any region of the W chromosome or not, we analyzed the presence or absence of 12 W-specific RAPD markers. The Df(p Sa + p W + od )Fem chromosome contained 3 of 12 W-specific RAPD markers. These results strongly indicate that the Df(p Sa + p W + od )Fem chromosome contains the region of the W chromosome. Moreover, by using phenotypic and molecular markers, we confirmed that the Df(p Sa + p W + od )Fem chromosome is connected with a partially deleted Z chromosome and that this fused chromosome behaves as a Z chromosome during male meiosis. Furthermore, we demonstrated that the ZZW-type triploid female having the Df(p Sa + p W + od )Fem chromosome is viable. Therefore, we concluded that the Df(p Sa + p W + od )Fem chromosome does not have a female-killing factor but that partial deletion of the Z chromosome causes the death of the ZW-type diploid female having the Df(p Sa + p W + od )Fem chromosome. Additionally, our results of detailed genetic analyses strongly indicate that the female-killing chromosome composed of the Df(p Sa + p W + od )Fem chromosome and deleted Z chromosome was generated by translocation between the Z chromosome and the translocation-carrying W chromosome, p Sa + p W + od.

Original languageEnglish
Pages (from-to)253-265
Number of pages13
JournalGenetica
Volume127
Issue number1-3
DOIs
Publication statusPublished - May 1 2006

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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    Fujii, T., Tanaka, N., Yokoyama, T., Ninaki, O., Oshiki, T., Ohnuma, A., Tazima, Y., Banno, Y., Ajimura, M., Mita, K., Seki, M., Ohbayashi, F., Shimada, T., & Abe, H. (2006). The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes. Genetica, 127(1-3), 253-265. https://doi.org/10.1007/s10709-005-4147-8