The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters

Yukiko Nakase, Mai Nakase, Jun Kashiwazaki, Tomoka Murai, Yoko Otsubo, Issei Mabuchi, Masayuki Yamamoto, Kaoru Takegawa, Tomohiro Matsumoto

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Abstract

Rheb GTPase and the Tsc1-Tsc2 protein complex, which serves as a GTPase-activating protein for Rheb, have crucial roles in the regulation of cell growth in response to extracellular conditions. In Schizosaccharomyces pombe, Rheb and Tsc1-Tsc2 regulate cell cycle progression, the onset of meiosis and the uptake of amino acids. In cells lacking Tsc2 (Dtsc2), the amino acid transporter Aat1, which is normally expressed on the plasma membrane under starvation conditions, is confined to the Golgi. Here, we show that the loss of either pub1+, encoding an E3 ubiquitin ligase, or any1+, encoding a b-arrestin-like protein, allows constitutive expression of Aat1 on the plasma membrane in Dtsc2 cells, suggesting that Pub1 and Any1 are required for localization of Aat1 to the Golgi. Subsequent analysis revealed that, in the Golgi, Pub1 and Any1 form a complex that ubiquitylates Aat1. Physical interaction of Pub1 and Any1 is more stable in Dtsc2 cells than in wild-type cells and is independent of Tor2 activity. These results indicate that the TSC-Rheb signaling pathway regulates the localization of amino acid transporters via Pub1 and Any1 in a Tor2-independent manner. Our study demonstrates that, unlike in budding yeast (in which Rsp5 and ARTs, a pair of proteins analogous to Pub1 and Any1, respectively, primarily act to reduce expression of the transporters on plasma membrane when nutrients are abundant), the primary role of fission yeast Pub1 and Any1 is to store the transporter in the Golgi under nutrient-rich conditions.

Original languageEnglish
Pages (from-to)3972-3981
Number of pages10
JournalJournal of Cell Science
Volume126
Issue number17
DOIs
Publication statusPublished - Sep 5 2013

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Arrestin
Amino Acid Transport Systems
Schizosaccharomyces
Cell Membrane
Proteins
GTPase-Activating Proteins
Food
Saccharomycetales
Ubiquitin-Protein Ligases
GTP Phosphohydrolases
Meiosis
Starvation
Cell Cycle
Amino Acids
Growth

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Nakase, Y., Nakase, M., Kashiwazaki, J., Murai, T., Otsubo, Y., Mabuchi, I., ... Matsumoto, T. (2013). The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters. Journal of Cell Science, 126(17), 3972-3981. https://doi.org/10.1242/jcs.128355

The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters. / Nakase, Yukiko; Nakase, Mai; Kashiwazaki, Jun; Murai, Tomoka; Otsubo, Yoko; Mabuchi, Issei; Yamamoto, Masayuki; Takegawa, Kaoru; Matsumoto, Tomohiro.

In: Journal of Cell Science, Vol. 126, No. 17, 05.09.2013, p. 3972-3981.

Research output: Contribution to journalArticle

Nakase, Y, Nakase, M, Kashiwazaki, J, Murai, T, Otsubo, Y, Mabuchi, I, Yamamoto, M, Takegawa, K & Matsumoto, T 2013, 'The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters', Journal of Cell Science, vol. 126, no. 17, pp. 3972-3981. https://doi.org/10.1242/jcs.128355
Nakase, Yukiko ; Nakase, Mai ; Kashiwazaki, Jun ; Murai, Tomoka ; Otsubo, Yoko ; Mabuchi, Issei ; Yamamoto, Masayuki ; Takegawa, Kaoru ; Matsumoto, Tomohiro. / The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters. In: Journal of Cell Science. 2013 ; Vol. 126, No. 17. pp. 3972-3981.
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